7K98

Preaminoacylation complex of M. tuberculosis PheRS with cognate precursor tRNA and 5'-O-(N-phenylalanyl)sulfamoyl-adenosine (F-AMS)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, D)	Phenylalanine--tRNA ligase alpha subunit	polymer	344	37687.5	2	UniProt (P9WFU3) Pfam (PF02912) Pfam (PF01409)	Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)	Phenylalanyl-tRNA synthetase alpha subunit,PheRS
2	B, D (B, E)	Phenylalanine--tRNA ligase beta subunit	polymer	840	89534.0	2	UniProt (P9WFU1) Pfam (PF01588) Pfam (PF03483) Pfam (PF03484) Pfam (PF17759) Pfam (PF03147)	Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)	Phenylalanyl-tRNA synthetase beta subunit,PheRS
3	E, F (C, F)	tRNA(Phe)	polymer	77	24842.8	2	GenBank (1251771558)	Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
4	G, O (A, D)	5'-O-(L-phenylalanylsulfamoyl)adenosine	non-polymer		493.5	2	Chemie (W5Y)
5	H, I, J, K, L... (A, B, D, C, F)	MAGNESIUM ION	non-polymer		24.3	13	Chemie (MG)
6	M, S (B, E)	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL)
7	N, T, W (B, E, C)	GLYCEROL	non-polymer		92.1	3	Chemie (GOL)
8	AA, BA, CA, DA, EA... (A, B, D, E, C...)	water	water		18.0	672	Chemie (HOH)

Sequence modifications

A, D: 1 - 341 (UniProt: P9WFU3)

PDB	External Database	Details
Ser -2	-	expression tag
Asn -1	-	expression tag
Ala 0	-	expression tag

B, E: 1 - 831 (UniProt: P9WFU1)

PDB	External Database	Details
Glu -8	-	expression tag
Asn -7	-	expression tag
Leu -6	-	expression tag
Tyr -5	-	expression tag
Phe -4	-	expression tag
Gln -3	-	expression tag
Ser -2	-	expression tag
Asn -1	-	expression tag
Ala 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	304128.6
Non-Polymers*	Number of molecules	20
	Total formula weight	1650.1
All*	Total formula weight	305778.7

*Water molecules are not included.