7K6P
Active state Dot1 bound to the unacetylated H4 nucleosome
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, E | Histone H3.2 | polymer | 98 | 11562.6 | 2 | UniProt (P84233) Pfam (PF00125) In PDB | Xenopus laevis (African clawed frog) | |
2 | B, F | Histone H4 | polymer | 90 | 10176.0 | 2 | UniProt (P62799) Pfam (PF15511) In PDB | Xenopus laevis (African clawed frog) | |
3 | C, G | Histone H2A type 1 | polymer | 107 | 11694.6 | 2 | UniProt (P06897) Pfam (PF00125) Pfam (PF16211) In PDB | Xenopus laevis (African clawed frog) | |
4 | D, H | Histone H2B 1.1 | polymer | 93 | 10322.8 | 2 | UniProt (P02281) Pfam (PF00125) In PDB | Xenopus laevis (African clawed frog) | H2B1.1 |
5 | I | DNA (146-MER) | polymer | 146 | 44824.6 | 1 | synthetic construct | ||
6 | J | DNA (146-MER) | polymer | 146 | 45304.9 | 1 | synthetic construct | ||
7 | K | Histone-lysine N-methyltransferase, H3 lysine-79 specific | polymer | 406 | 46686.1 | 1 | UniProt (Q04089) Pfam (PF08123) In PDB | Saccharomyces cerevisiae (Baker's yeast) | Disrupter of telomere silencing protein 1,Histone H3-K79 methyltransferase,H3-K79-HMTase,Lysine N-methyltransferase 4 |
8 | L | Ubiquitin | polymer | 76 | 8622.9 | 1 | UniProt (P0CG48) Pfam (PF00240) In PDB | Homo sapiens (Human) | |
9 | K | S-ADENOSYLMETHIONINE | non-polymer | 398.4 | 1 | Chemie (SAM) |
Sequence modifications
A, E: 37 - 134 (UniProt: P84233)
C, G: 12 - 118 (UniProt: P06897)
D, H: 32 - 124 (UniProt: P02281)
K: 176 - 580 (UniProt: Q04089)
L: 1 - 76 (UniProt: P0CG48)
PDB | External Database | Details |
---|---|---|
Met 79 | Lys 80 | engineered mutation |
Ala 102 | Gly 103 | engineered mutation |
PDB | External Database | Details |
---|---|---|
Arg 99 | Gly 100 | engineered mutation |
PDB | External Database | Details |
---|---|---|
Thr 32 | Ser 33 | engineered mutation |
Cys 120 | Lys 121 | engineered mutation |
PDB | External Database | Details |
---|---|---|
Met 581 | - | expression tag |
PDB | External Database | Details |
---|---|---|
Cys 76 | Gly 76 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 12 |
Total formula weight | 232950.4 | |
Non-Polymers* | Number of molecules | 1 |
Total formula weight | 398.4 | |
All* | Total formula weight | 233348.8 |