7K6P
Active state Dot1 bound to the unacetylated H4 nucleosome
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, E (A, E) | Histone H3.2 | polymer | 98 | 11562.6 | 2 | UniProt (P84233) Pfam (PF00125) | Xenopus laevis (African clawed frog) | |
| 2 | B, F (B, F) | Histone H4 | polymer | 90 | 10176.0 | 2 | UniProt (P62799) Pfam (PF15511) | Xenopus laevis (African clawed frog) | |
| 3 | C, G (C, G) | Histone H2A type 1 | polymer | 107 | 11694.6 | 2 | UniProt (P06897) Pfam (PF00125) Pfam (PF16211) | Xenopus laevis (African clawed frog) | |
| 4 | D, H (D, H) | Histone H2B 1.1 | polymer | 93 | 10322.8 | 2 | UniProt (P02281) Pfam (PF00125) | Xenopus laevis (African clawed frog) | H2B1.1 |
| 5 | I (I) | DNA (146-MER) | polymer | 146 | 44824.6 | 1 | synthetic construct | ||
| 6 | J (J) | DNA (146-MER) | polymer | 146 | 45304.9 | 1 | synthetic construct | ||
| 7 | K (K) | Histone-lysine N-methyltransferase, H3 lysine-79 specific | polymer | 406 | 46686.1 | 1 | UniProt (Q04089) Pfam (PF08123) | Saccharomyces cerevisiae (Baker's yeast) | Disrupter of telomere silencing protein 1,Histone H3-K79 methyltransferase,H3-K79-HMTase,Lysine N-methyltransferase 4 |
| 8 | L (L) | Ubiquitin | polymer | 76 | 8622.9 | 1 | UniProt (P0CG48) Pfam (PF00240) | Homo sapiens (Human) | |
| 9 | M (K) | S-ADENOSYLMETHIONINE | non-polymer | 398.4 | 1 | Chemie (SAM) PubChem (5136) PubChem (34755) PubChem (58633578) PubChem (60038769) PubChem (73897310) PubChem (58766358) PubChem (87224684) PubChem (131697735) PubChem (9865603) PubChem (145712080) PubChem (12312798) PubChem (132988922) PubChem (134692588) PubChem (135030730) PubChem (140459785) PubChem (163046094) |
Sequence modifications
A, E: 37 - 134 (UniProt: P84233)
C, G: 12 - 118 (UniProt: P06897)
D, H: 32 - 124 (UniProt: P02281)
K: 176 - 580 (UniProt: Q04089)
L: 1 - 76 (UniProt: P0CG48)
| PDB | External Database | Details |
|---|---|---|
| Met 79 | Lys 80 | engineered mutation |
| Ala 102 | Gly 103 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Arg 99 | Gly 100 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Thr 32 | Ser 33 | engineered mutation |
| Cys 120 | Lys 121 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Met 581 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Cys 76 | Gly 76 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 12 |
| Total formula weight | 232950.4 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 398.4 | |
| All* | Total formula weight | 233348.8 |
