7K14

Ternary soak structure of alkanesulfonate monooxygenase MsuD from Pseudomonas fluorescens with FMN and methanesulfonate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E... (A, B, C, D, E...)	Alkanesulfonate monooxygenase	polymer	404	44278.9	8	UniProt (Q3K9A1) Pfam (PF00296)	Pseudomonas fluorescens	FMNH2-dependent aliphatic sulfonate monooxygenase, flavin-dependent methanesulfonate monooxygenase
2	I, K, M, O, Q... (A, B, C, D, E...)	FLAVIN MONONUCLEOTIDE	non-polymer		456.3	7	Chemie (FMN)
3	J, L, N, P, R (A, B, C, D, E)	methanesulfonic acid	non-polymer		96.1	5	Chemie (03S)
4	S (F)	PHOSPHATE ION	non-polymer		95.0	1	Chemie (PO4)
5	U, W (G, H)	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL)
6	AA, BA, CA, X, Y... (D, E, F, A, B...)	water	water		18.0	16	Chemie (HOH)

Sequence modifications

A, B, C, D, E, F, G, H: 1 - 381 (UniProt: Q3K9A1)

PDB	External Database	Details
Met -22	-	initiating methionine
Gly -21	-	expression tag
Ser -20	-	expression tag
Ser -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
Ser -12	-	expression tag
Ser -11	-	expression tag
Gly -10	-	expression tag
Leu -9	-	expression tag
Val -8	-	expression tag
Pro -7	-	expression tag
Arg -6	-	expression tag
Gly -5	-	expression tag
Ser -4	-	expression tag
His -3	-	expression tag
Met -2	-	expression tag
Ala -1	-	expression tag
Ser 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	354231.1
Non-Polymers*	Number of molecules	15
	Total formula weight	3840.8
All*	Total formula weight	358071.9

*Water molecules are not included.