7K0B
The internal aldimine form of Salmonella typhimurium Tryptophan Synthase mutant beta-Q114A with cesium ion at the metal coordination site. A random beta-P270L mutation was inserted during PCR step
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Tryptophan synthase alpha chain | polymer | 268 | 28698.8 | 1 | UniProt (P00929) Pfam (PF00290) | Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) | |
| 2 | B (B) | Tryptophan synthase beta chain | polymer | 397 | 42877.9 | 1 | UniProt (P0A2K1) Pfam (PF00291) | Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) | |
| 3 | C, D, F, G, R... (A, B) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 8 | Chemie (EDO) | |||
| 4 | E (A) | SERINE | non-polymer | 105.1 | 1 | Chemie (SER) | |||
| 5 | H (A) | DI(HYDROXYETHYL)ETHER | non-polymer | 106.1 | 1 | Chemie (PEG) | |||
| 6 | AA, BA, CA, DA, EA... (B, A) | DIMETHYL SULFOXIDE | non-polymer | 78.1 | 17 | Chemie (DMS) | |||
| 7 | KA, LA, MA, NA, O... (B, A) | CHLORIDE ION | non-polymer | 35.5 | 7 | Chemie (CL) | |||
| 8 | Q (B) | PYRIDOXAL-5'-PHOSPHATE | non-polymer | 247.1 | 1 | Chemie (PLP) | |||
| 9 | U (B) | GLYCEROL | non-polymer | 92.1 | 1 | Chemie (GOL) | |||
| 10 | W (B) | BICINE | non-polymer | 163.2 | 1 | Chemie (BCN) | |||
| 11 | IA, JA (B) | CESIUM ION | non-polymer | 132.9 | 2 | Chemie (CS) | |||
| 12 | PA, QA (A, B) | water | water | 18.0 | 677 | Chemie (HOH) |
Sequence modifications
B: 1 - 397 (UniProt: P0A2K1)
| PDB | External Database | Details |
|---|---|---|
| Ala 114 | Gln 114 | engineered mutation |
| Leu 270 | Pro 270 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 71576.7 | |
| Non-Polymers* | Number of molecules | 39 |
| Total formula weight | 3052.4 | |
| All* | Total formula weight | 74629.1 |
