7JZZ

Cryo-EM structure of CRISPR-Cas surveillance complex with AcrIF14

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	CRISPR-associated protein Csy1	polymer	434	49194.2	1	UniProt (Q02ML9) Pfam (PF09611) In PDB	Pseudomonas aeruginosa	Csy1 (Cas8f)
2	B	Type I-F CRISPR-associated protein Csy2	polymer	327	36244.1	1	UniProt (B3G161) Pfam (PF09614) UniProt (by SIFTS) (Q02MM0) In PDB	Pseudomonas aeruginosa	Csy2 (Cas5f)
3	C	Type I-F CRISPR-associated endoribonuclease Cas6/Csy4	polymer	189	21675.8	1	UniProt (A0A643HZS6) Pfam (PF09618) UniProt (by SIFTS) (Q02MM2) In PDB	Pseudomonas aeruginosa
4	D, E, F, G, H...	CRISPR type I-F/YPEST-associated protein Csy3	polymer	342	37579.3	6	UniProt (A0A444M080) Pfam (PF09615) UniProt (by SIFTS) (Q02MM1) In PDB	Pseudomonas aeruginosa	CRISPR-associated protein Csy3
5	J, K	AcrF14	polymer	124	14297.4	2	UniProt (by SIFTS) (A0A0R6PCL0) In PDB	unidentified
6	M	RNA (61-MER)	polymer	61	19538.6	1	GenBank (313291946)	Pseudomonas aeruginosa

Sequence modifications

C: 1 - 187 (UniProt: A0A643HZS6)

PDB	External Database	Details
Phe -1	-	expression tag
Thr 0	-	expression tag

M: 1 - 61 (GenBank: 313291946)

PDB	External Database	Details
A 41	G 1458	conflict
A 53	G 1446	conflict

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	380723.0
All*	Total formula weight	380723.0

*Water molecules are not included.