7JZW
Cryo-EM structure of CRISPR-Cas surveillance complex with AcrIF4
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | CRISPR type I-F/YPEST-associated protein Csy1 | polymer | 434 | 49136.1 | 1 | UniProt (A0A643HYU6) Pfam (PF09611) UniProt (by SIFTS) (Q02ML9) In PDB | Pseudomonas aeruginosa | Type I-F CRISPR-associated protein Csy1 |
2 | C | CRISPR-associated endonuclease Cas6/Csy4 | polymer | 187 | 21427.5 | 1 | UniProt (Q02MM2) Pfam (PF09618) In PDB | Pseudomonas aeruginosa | Csy4 (Cas6f) |
3 | B | CRISPR type I-F/YPEST-associated protein Csy2 | polymer | 329 | 36446.4 | 1 | UniProt (B3G161) Pfam (PF09614) UniProt (by SIFTS) (Q02MM0) In PDB | Pseudomonas aeruginosa | Type I-F CRISPR-associated protein Csy2 |
4 | E, D, F, G, H... | CRISPR type I-F/YPEST-associated protein Csy3 | polymer | 344 | 37781.5 | 6 | UniProt (A0A444M080) Pfam (PF09615) UniProt (by SIFTS) (Q02MM1) In PDB | Pseudomonas aeruginosa | CRISPR-associated protein Csy3 |
5 | J | Type I-F anti-CRISPR protein | polymer | 100 | 11087.5 | 1 | UniProt (A0A076FR21) UniProt (by SIFTS) (L7P7U3) In PDB | Pseudomonas phage sp. | |
6 | M | CRISPR repeat sequence | polymer | 61 | 19538.6 | 1 | GenBank (313291946) | Pseudomonas aeruginosa |
Sequence modifications
A: 1 - 434 (UniProt: A0A643HYU6)
B: 1 - 327 (UniProt: B3G161)
E, D, F, G, H, I: 20 - 361 (UniProt: A0A444M080)
M: 1 - 61 (GenBank: 313291946)
PDB | External Database | Details |
---|---|---|
Ala 288 | Glu 288 | conflict |
PDB | External Database | Details |
---|---|---|
Met -1 | - | expression tag |
Ala 0 | - | expression tag |
PDB | External Database | Details |
---|---|---|
Met 18 | - | expression tag |
Ala 19 | - | expression tag |
PDB | External Database | Details |
---|---|---|
A 41 | G 1458 | conflict |
A 53 | G 1446 | conflict |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 11 |
Total formula weight | 364325.4 | |
All* | Total formula weight | 364325.4 |