7JZW

Cryo-EM structure of CRISPR-Cas surveillance complex with AcrIF4

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	CRISPR type I-F/YPEST-associated protein Csy1	polymer	434	49136.1	1	UniProt (A0A643HYU6) Pfam (PF09611) UniProt (by SIFTS) (Q02ML9) In PDB	Pseudomonas aeruginosa	Type I-F CRISPR-associated protein Csy1
2	C	CRISPR-associated endonuclease Cas6/Csy4	polymer	187	21427.5	1	UniProt (Q02MM2) Pfam (PF09618) In PDB	Pseudomonas aeruginosa	Csy4 (Cas6f)
3	B	CRISPR type I-F/YPEST-associated protein Csy2	polymer	329	36446.4	1	UniProt (B3G161) Pfam (PF09614) UniProt (by SIFTS) (Q02MM0) In PDB	Pseudomonas aeruginosa	Type I-F CRISPR-associated protein Csy2
4	E, D, F, G, H...	CRISPR type I-F/YPEST-associated protein Csy3	polymer	344	37781.5	6	UniProt (A0A444M080) Pfam (PF09615) UniProt (by SIFTS) (Q02MM1) In PDB	Pseudomonas aeruginosa	CRISPR-associated protein Csy3
5	J	Type I-F anti-CRISPR protein	polymer	100	11087.5	1	UniProt (A0A076FR21) UniProt (by SIFTS) (L7P7U3) In PDB	Pseudomonas phage sp.
6	M	CRISPR repeat sequence	polymer	61	19538.6	1	GenBank (313291946)	Pseudomonas aeruginosa

Sequence modifications

A: 1 - 434 (UniProt: A0A643HYU6)

PDB	External Database	Details
Ala 288	Glu 288	conflict

B: 1 - 327 (UniProt: B3G161)

PDB	External Database	Details
Met -1	-	expression tag
Ala 0	-	expression tag

E, D, F, G, H, I: 20 - 361 (UniProt: A0A444M080)

PDB	External Database	Details
Met 18	-	expression tag
Ala 19	-	expression tag

M: 1 - 61 (GenBank: 313291946)

PDB	External Database	Details
A 41	G 1458	conflict
A 53	G 1446	conflict

Contents of the asymmetric unit

Polymers	Number of chains	11
	Total formula weight	364325.4
All*	Total formula weight	364325.4

*Water molecules are not included.