7JVV

Crystal structure of human histone deacetylase 8 (HDAC8) E66D/Y306F double mutation complexed with a tetrapeptide substrate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Histone deacetylase 8	polymer	389	43202.0	2	UniProt (Q9BY41) Pfam (PF00850)	Homo sapiens (Human)	HD8
2	C, D (C, D)	ACE-ARG-HIS-ALY-ALY-MCM	polymer	6	837.0	2		Homo sapiens (Human)
3	E, F, G, L, M... (A, B, C, D)	1,2-ETHANEDIOL	non-polymer		62.1	8	Chemie (EDO)
4	H, O (A, B)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
5	I, J, P, Q (A, B)	POTASSIUM ION	non-polymer		39.1	4	Chemie (K)
6	K, R (A, B)	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
7	U, V, W, X (A, B, C, D)	water	water		18.0	447	Chemie (HOH)

Sequence modifications

A, B: 1 - 377 (UniProt: Q9BY41)

PDB	External Database	Details
Asp 66	Glu 66	engineered mutation
Phe 306	Tyr 306	engineered mutation
Ile 378	-	expression tag
Glu 379	-	expression tag
Gly 380	-	expression tag
Arg 381	-	expression tag
Gly 382	-	expression tag
Ser 383	-	expression tag
His 384	-	expression tag
His 385	-	expression tag
His 386	-	expression tag
His 387	-	expression tag
His 388	-	expression tag
His 389	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	88077.9
Non-Polymers*	Number of molecules	16
	Total formula weight	967.9
All*	Total formula weight	89045.8

*Water molecules are not included.