7JVQ

Cryo-EM structure of apomorphine-bound dopamine receptor 1 in complex with Gs protein

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (R)	D(1A) dopamine receptor	polymer	502	55668.7	1	UniProt (P21728) Pfam (PF00001)	Homo sapiens (Human)	Dopamine D1 receptor
2	B (A)	Engineered mini-Gi protein alpha sub-unit	polymer	246	29048.9	1	Pfam (PF00503) UniProt (by SIFTS) (P63092)	Homo sapiens (human)
3	C (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	354	39077.7	1	UniProt (P62873) Pfam (PF00400)	Homo sapiens (Human)	Transducin beta chain 1
4	D (G)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	67	7432.6	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (Human)	G gamma-I
5	E (N)	Nanobody35	polymer	135	14845.5	1		synthetic construct
6	F (R)	(6aR)-6-methyl-5,6,6a,7-tetrahydro-4H-dibenzo[de,g]quinoline-10,11-diol	non-polymer		267.3	1	Chemie (OR9)
7	G, H, I, J, K (R)	CHOLESTEROL	non-polymer		386.7	5	Chemie (CLR)
8	L, M, N (R)	PALMITIC ACID	non-polymer		256.4	3	Chemie (PLM)

Sequence modifications

R: 1 - 446 (UniProt: P21728)

PDB	External Database	Details
Asp -47	-	expression tag
Tyr -46	-	expression tag
Lys -45	-	expression tag
Asp -44	-	expression tag
Asp -43	-	expression tag
Asp -42	-	expression tag
Asp -41	-	expression tag
Val -40	-	expression tag
Asp -39	-	expression tag
Met -38	-	expression tag
Gly -37	-	expression tag
Gln -36	-	expression tag
Pro -35	-	expression tag
Gly -34	-	expression tag
Asn -33	-	expression tag
Gly -32	-	expression tag
Ser -31	-	expression tag
Ala -30	-	expression tag
Phe -29	-	expression tag
Leu -28	-	expression tag
Leu -27	-	expression tag
Ala -26	-	expression tag
Pro -25	-	expression tag
Asn -24	-	expression tag
Gly -23	-	expression tag
Ser -22	-	expression tag
His -21	-	expression tag
Ala -20	-	expression tag
Pro -19	-	expression tag
Asp -18	-	expression tag
His -17	-	expression tag
Asp -16	-	expression tag
Val -15	-	expression tag
Thr -14	-	expression tag
Gln -13	-	expression tag
Gln -12	-	expression tag
Arg -11	-	expression tag
Asp -10	-	expression tag
Glu -9	-	expression tag
Glu -8	-	expression tag
Asn -7	-	expression tag
Leu -6	-	expression tag
Tyr -5	-	expression tag
Phe -4	-	expression tag
Gln -3	-	expression tag
Gly -2	-	expression tag
Ala -1	-	expression tag
Ser 0	-	expression tag
His 447	-	expression tag
His 448	-	expression tag
His 449	-	expression tag
His 450	-	expression tag
His 451	-	expression tag
His 452	-	expression tag
His 453	-	expression tag
His 454	-	expression tag

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
Met -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Leu -1	-	expression tag
Leu 0	-	expression tag
Gln 1	-	expression tag
Gly 341	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	146073.4
Non-Polymers*	Number of molecules	9
	Total formula weight	2969.9
All*	Total formula weight	149043.3

*Water molecules are not included.