7JHH
Cryo-EM structure of ATP-bound fully inactive AMPK in complex with Fab and nanobody
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | 5'-AMP-activated protein kinase catalytic subunit alpha-1 | polymer | 484 | 56004.4 | 1 | UniProt (Q13131) Pfam (PF00069) Pfam (PF21147) Pfam (PF16579) | Homo sapiens (Human) | AMPK subunit alpha-1,Acetyl-CoA carboxylase kinase,ACACA kinase,Hydroxymethylglutaryl-CoA reductase kinase,HMGCR kinase,Tau-protein kinase PRKAA1 |
| 2 | B (B) | 5'-AMP-activated protein kinase subunit beta-2 | polymer | 198 | 22384.7 | 1 | UniProt (O43741) Pfam (PF16561) Pfam (PF04739) | Homo sapiens (Human) | AMPK subunit beta-2 |
| 3 | C (G) | 5'-AMP-activated protein kinase subunit gamma-1 | polymer | 306 | 34833.4 | 1 | UniProt (P54619) Pfam (PF00571) | Homo sapiens (Human) | AMPKg |
| 4 | D (M) | Maltodextrin-binding protein | polymer | 373 | 40827.1 | 1 | UniProt (C3SHQ8) Pfam (PF01547) UniProt (by SIFTS) (P0AEX9) | Escherichia coli K-12 | |
| 5 | E (L) | Fab light chain | polymer | 215 | 23212.7 | 1 | synthetic construct | ||
| 6 | F (H) | Fab heavy chain | polymer | 237 | 25483.5 | 1 | synthetic construct | ||
| 7 | G (N) | Nanobody | polymer | 159 | 17414.4 | 1 | synthetic construct | ||
| 8 | H (C) | alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose | branched | 342.3 | 1 | In PDB BIRD (PRD_900001) GlyTouCan (G07411ON) | |||
| 9 | I (G) | ADENOSINE-5'-TRIPHOSPHATE | non-polymer | 507.2 | 1 | Chemie (ATP) | |||
| 10 | J (G) | ADENOSINE-5'-DIPHOSPHATE | non-polymer | 427.2 | 1 | Chemie (ADP) | |||
| 11 | K (G) | ADENOSINE MONOPHOSPHATE | non-polymer | 347.2 | 1 | Chemie (AMP) |
Sequence modifications
A: 13 - 471 (UniProt: Q13131)
A: 472 - 550 (UniProt: Q13131)
B: 75 - 272 (UniProt: O43741)
G: 24 - 327 (UniProt: P54619)
M: 0 - 366 (UniProt: C3SHQ8)
A: 472 - 550 (UniProt: Q13131)
B: 75 - 272 (UniProt: O43741)
| PDB | External Database | Details |
|---|---|---|
| Met 75 | Gln 75 | conflict |
| Ala 199 | Glu 199 | conflict |
| Ala 200 | Glu 200 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Met 22 | - | expression tag |
| Gly 23 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Met -1 | - | initiating methionine |
| Ala 82 | Asp 108 | conflict |
| Ala 83 | Lys 109 | conflict |
| Ala 239 | Lys 265 | conflict |
| Asn 367 | - | expression tag |
| Ala 368 | - | expression tag |
| Ala 369 | - | expression tag |
| Glu 370 | - | expression tag |
| Phe 371 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 7 |
| Total formula weight | 220160.1 | |
| Branched | Number of molecules | 1 |
| Total formula weight | 342.3 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 1281.6 | |
| All* | Total formula weight | 221784.0 |
