7JHH
Cryo-EM structure of ATP-bound fully inactive AMPK in complex with Fab and nanobody
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | 5'-AMP-activated protein kinase catalytic subunit alpha-1 | polymer | 484 | 56004.4 | 1 | UniProt (Q13131) Pfam (PF00069) Pfam (PF21147) Pfam (PF16579) | Homo sapiens (Human) | AMPK subunit alpha-1,Acetyl-CoA carboxylase kinase,ACACA kinase,Hydroxymethylglutaryl-CoA reductase kinase,HMGCR kinase,Tau-protein kinase PRKAA1 |
| 2 | B (B) | 5'-AMP-activated protein kinase subunit beta-2 | polymer | 198 | 22384.7 | 1 | UniProt (O43741) Pfam (PF16561) Pfam (PF04739) | Homo sapiens (Human) | AMPK subunit beta-2 |
| 3 | C (G) | 5'-AMP-activated protein kinase subunit gamma-1 | polymer | 306 | 34833.4 | 1 | UniProt (P54619) Pfam (PF00571) | Homo sapiens (Human) | AMPKg |
| 4 | D (M) | Maltodextrin-binding protein | polymer | 373 | 40827.1 | 1 | UniProt (C3SHQ8) Pfam (PF01547) UniProt (by SIFTS) (P0AEX9) | Escherichia coli K-12 | |
| 5 | E (L) | Fab light chain | polymer | 215 | 23212.7 | 1 | synthetic construct | ||
| 6 | F (H) | Fab heavy chain | polymer | 237 | 25483.5 | 1 | synthetic construct | ||
| 7 | G (N) | Nanobody | polymer | 159 | 17414.4 | 1 | synthetic construct | ||
| 8 | H (C) | alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose | branched | 342.3 | 1 | In PDB BIRD (PRD_900001) GlyTouCan (G07411ON) | |||
| 9 | I (G) | ADENOSINE-5'-TRIPHOSPHATE | non-polymer | 507.2 | 1 | Chemie (ATP) | |||
| 10 | J (G) | ADENOSINE-5'-DIPHOSPHATE | non-polymer | 427.2 | 1 | Chemie (ADP) | |||
| 11 | K (G) | ADENOSINE MONOPHOSPHATE | non-polymer | 347.2 | 1 | Chemie (AMP) PubChem (16397049) PubChem (16760266) PubChem (224) PubChem (40467049) PubChem (40469468) PubChem (34768) PubChem (44348067) PubChem (83862) PubChem (45358029) PubChem (46874558) PubChem (40459109) PubChem (57358045) PubChem (57879893) PubChem (57879902) PubChem (46875232) PubChem (446048) PubChem (515384) PubChem (638042) PubChem (177678969) PubChem (6083) PubChem (1549371) PubChem (5316444) PubChem (92144436) PubChem (5742864) PubChem (122197642) PubChem (12358354) PubChem (12163610) PubChem (12358355) PubChem (162290139) PubChem (162946266) PubChem (171699505) |
Sequence modifications
A: 13 - 471 (UniProt: Q13131)
A: 472 - 550 (UniProt: Q13131)
B: 75 - 272 (UniProt: O43741)
G: 24 - 327 (UniProt: P54619)
M: 0 - 366 (UniProt: C3SHQ8)
A: 472 - 550 (UniProt: Q13131)
B: 75 - 272 (UniProt: O43741)
| PDB | External Database | Details |
|---|---|---|
| Met 75 | Gln 75 | conflict |
| Ala 199 | Glu 199 | conflict |
| Ala 200 | Glu 200 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Met 22 | - | expression tag |
| Gly 23 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Met -1 | - | initiating methionine |
| Ala 82 | Asp 108 | conflict |
| Ala 83 | Lys 109 | conflict |
| Ala 239 | Lys 265 | conflict |
| Asn 367 | - | expression tag |
| Ala 368 | - | expression tag |
| Ala 369 | - | expression tag |
| Glu 370 | - | expression tag |
| Phe 371 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 7 |
| Total formula weight | 220160.1 | |
| Branched | Number of molecules | 1 |
| Total formula weight | 342.3 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 1281.6 | |
| All* | Total formula weight | 221784.0 |
