7FRW

Structure of liver pyruvate kinase in complex with allosteric modulator 4

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E... (A, B, C, D, E...)	Pyruvate kinase PKLR	polymer	447	48285.4	8	UniProt (P30613) Pfam (PF00224) Pfam (PF02887)	Homo sapiens (human)	Pyruvate kinase 1,Pyruvate kinase isozymes L/R,R-type/L-type pyruvate kinase,Red cell/liver pyruvate kinase
2	CA, HA, I, MA, N... (E, F, A, G, B...)	1,6-di-O-phosphono-beta-D-fructofuranose	non-polymer		340.1	8	Chemie (FBP)
3	DA, IA, J, NA, O... (E, F, A, G, B...)	OXALATE ION	non-polymer		88.0	8	Chemie (OXL)
4	EA, JA, K, OA, P... (E, F, A, G, B...)	MAGNESIUM ION	non-polymer		24.3	8	Chemie (MG)
5	AA, FA, KA, L, PA... (D, E, F, A, G...)	POTASSIUM ION	non-polymer		39.1	8	Chemie (K)
6	BA, GA, LA, M, QA... (D, E, F, A, G...)	(10aM)-3,8,9-trihydroxy-6H-6lambda~6~-dibenzo[c,e][1,2]oxathiine-6,6-dione	non-polymer		280.3	8	Chemie (OA0)
7	AB, BB, CB, DB, WA... (E, F, G, H, A...)	water	water		18.0	2735	Chemie (HOH)

Sequence modifications

A, B, C, D, E, F, G, H: 3 - 129 (UniProt: P30613)

PDB	External Database	Details
Gly -1	-	expression tag
Ser 0	-	expression tag
Met 1	-	expression tag
Glu 2	-	expression tag
Asp 12	Ser 43	conflict

A, B, C, D, E, F, G, H: 231 - 543 (UniProt: P30613)

PDB	External Database	Details
Gly 130	-	linker
Ser 131	-	linker
Gly 132	-	linker

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	386283.0
Non-Polymers*	Number of molecules	40
	Total formula weight	6174.3
All*	Total formula weight	392457.4

*Water molecules are not included.