7FJN

Cryo-EM structure of South African (B.1.351) SARS-CoV-2 spike glycoprotein in complex with two T6 Fab

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C (A, B, C)	Spike glycoprotein,Envelope glycoprotein	polymer	1280	141515.1	3	UniProt (P0DTC2) UniProt (M1E1E4) Pfam (PF16451) Pfam (PF09408) Pfam (PF19209) Pfam (PF01601) Pfam (PF07921)	Severe acute respiratory syndrome coronavirus 2	S glycoprotein,E2,Peplomer protein
2	D, E (H, I)	T6 heavy chain	polymer	117	12833.1	2		Homo sapiens
3	F, G (J, L)	T6 light chain	polymer	113	12572.1	2		Homo sapiens
4	AA, BA, CA, DA, EA... (C, A, B)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	25	Chemie (NAG)

Sequence modifications

A, B, C: 16 - 1208 (UniProt: P0DTC2)

PDB	External Database	Details
Phe 18	Leu 18	variant
Ala 80	Asp 80	variant
Gly 215	Asp 215	variant
-	Leu 241	deletion
-	Leu 242	deletion
-	Ala 243	deletion
Thr 305	Ser 305	engineered mutation
Asn 417	Lys 417	variant
Lys 484	Glu 484	variant
Tyr 501	Asn 501	variant
Gly 614	Asp 614	variant
Gly 682	Arg 682	engineered mutation
Ser 683	Arg 683	engineered mutation
Ser 685	Arg 685	engineered mutation
Val 701	Ala 701	variant
Pro 986	Lys 986	engineered mutation
Pro 987	Val 987	engineered mutation
Gly 1209	-	linker
Ser 1210	-	linker

A, B, C: 1211 - 1238 (UniProt: M1E1E4)

PDB	External Database	Details
Arg 1239	-	expression tag
Ser 1240	-	expression tag
Leu 1241	-	expression tag
Glu 1242	-	expression tag
Val 1243	-	expression tag
Leu 1244	-	expression tag
Phe 1245	-	expression tag
Gln 1246	-	expression tag
Gly 1247	-	expression tag
Pro 1248	-	expression tag
Gly 1249	-	expression tag
His 1250	-	expression tag
His 1251	-	expression tag
His 1252	-	expression tag
His 1253	-	expression tag
His 1254	-	expression tag
His 1255	-	expression tag
His 1256	-	expression tag
His 1257	-	expression tag
Ser 1258	-	expression tag
Ala 1259	-	expression tag
Trp 1260	-	expression tag
Ser 1261	-	expression tag
His 1262	-	expression tag
Pro 1263	-	expression tag
Gln 1264	-	expression tag
Phe 1265	-	expression tag
Glu 1266	-	expression tag
Lys 1267	-	expression tag
Gly 1268	-	expression tag
Gly 1269	-	expression tag
Gly 1270	-	expression tag
Ser 1271	-	expression tag
Gly 1272	-	expression tag
Gly 1273	-	expression tag
Gly 1274	-	expression tag
Gly 1275	-	expression tag
Ser 1276	-	expression tag
Gly 1277	-	expression tag
Gly 1278	-	expression tag
Ser 1279	-	expression tag
Ala 1280	-	expression tag
Trp 1281	-	expression tag
Ser 1282	-	expression tag
His 1283	-	expression tag
Pro 1284	-	expression tag
Gln 1285	-	expression tag
Phe 1286	-	expression tag
Glu 1287	-	expression tag
Lys 1288	-	expression tag
Gly 1289	-	expression tag
Ser 1290	-	expression tag
Asp 1291	-	expression tag
Tyr 1292	-	expression tag
Lys 1293	-	expression tag
Asp 1294	-	expression tag
Asp 1295	-	expression tag
Asp 1296	-	expression tag
Asp 1297	-	expression tag
Lys 1298	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	7
	Total formula weight	475355.5
Non-Polymers*	Number of molecules	25
	Total formula weight	5530.2
All*	Total formula weight	480885.7

*Water molecules are not included.