7FI9

Crystal structure of human MICA mutants in complex with natural killer cell receptor NKG2D

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	NKG2-D type II integral membrane protein	polymer	139	16110.2	2	UniProt (P26718) Pfam (PF00059)	Homo sapiens (Human)	Killer cell lectin-like receptor subfamily K member 1,NK cell receptor D,NKG2-D-activating NK receptor
2	C (C)	MHC class I polypeptide-related sequence A	polymer	275	31885.6	1	UniProt (Q29983) Pfam (PF00129) Pfam (PF07654)	Homo sapiens (Human)	MIC-A
3	D, E (B, C)	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
4	F, G, H (A, B, C)	water	water		18.0	148	Chemie (HOH)

Sequence modifications

A, B: 80 - 216 (UniProt: P26718)

PDB	External Database	Details
Met 78	-	initiating methionine
Glu 79	-	expression tag

C: 1 - 274 (UniProt: Q29983)

PDB	External Database	Details
Met 0	-	initiating methionine
Asp 8	Asn 31	engineered mutation
Phe 9	Leu 32	engineered mutation
Ile 34	Leu 57	engineered mutation
His 108	Gln 131	engineered mutation
Trp 120	Gln 143	engineered mutation
Phe 127	Trp 150	engineered mutation
Trp 146	Leu 169	engineered mutation
Phe 157	Tyr 180	engineered mutation
Arg 161	His 184	engineered mutation
Ile 177	Val 200	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	64106.1
Non-Polymers*	Number of molecules	2
	Total formula weight	184.2
All*	Total formula weight	64290.2

*Water molecules are not included.