7FHO

Structure of AtTPC1 D240A/D454A/E528A mutant with 50 mM Ca2+

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, C)	Two pore calcium channel protein 1,GFP	polymer	998	114498.3	2	UniProt (Q94KI8) UniProt (A0A5P9VSM6) Pfam (PF00520) Pfam (PF01353) UniProt (by SIFTS) (P42212)	Arabidopsis thaliana (Mouse-ear cress)	Calcium channel protein 1,AtCCH1,Fatty acid oxygenation up-regulated protein 2,Voltage-dependent calcium channel protein TPC1,AtTPC1
2	C, D, E, F, G... (A, C)	CALCIUM ION	non-polymer		40.1	6	Chemie (CA)

Sequence modifications

A, C: 1 - 733 (UniProt: Q94KI8)

PDB	External Database	Details
Ala 240	Asp 240	engineered mutation
Ala 454	Asp 454	engineered mutation
Ala 528	Glu 528	engineered mutation
Ser 734	-	linker
Thr 735	-	linker
Ala 736	-	linker
Gly 737	-	linker
Leu 738	-	linker
Val 739	-	linker
Pro 740	-	linker
Arg 741	-	linker
Gly 742	-	linker
Ser 743	-	linker
Ala 744	-	linker
Ala 745	-	linker
Ala 746	-	linker
Ala 747	-	linker

A, C: 748 - 985 (UniProt: A0A5P9VSM6)

PDB	External Database	Details
Ser 986	-	expression tag
Gly 987	-	expression tag
Leu 988	-	expression tag
Arg 989	-	expression tag
Ser 990	-	expression tag
His 991	-	expression tag
His 992	-	expression tag
His 993	-	expression tag
His 994	-	expression tag
His 995	-	expression tag
His 996	-	expression tag
His 997	-	expression tag
His 998	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	228996.5
Non-Polymers*	Number of molecules	6
	Total formula weight	240.5
All*	Total formula weight	229237.0

*Water molecules are not included.