7FFN

Cryo-EM structure of VEEV VLP-LDLRAD3-D1 complex at the 5-fold axes

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	K	Capsid protein	polymer	275	30980.8	1	UniProt (P05674) Pfam (PF00944) In PDB	Venezuelan equine encephalitis virus (strain TC-83) (VEEV)	Coat protein,C
2	L	assembly protein E3	polymer	59	6488.6	1	UniProt (P05674) Pfam (PF01563) In PDB	Venezuelan equine encephalitis virus (strain TC-83) (VEEV)	p130
3	M	Low-density lipoprotein receptor class A domain-containing protein 3	polymer	80	8652.9	1	UniProt (Q86YD5) Pfam (PF00057) In PDB	Homo sapiens (Human)	LDLR class A domain-containing protein 3
4	N	Spike glycoprotein E2	polymer	423	47113.7	1	UniProt (P05674) Pfam (PF00943) In PDB	Venezuelan equine encephalitis virus (strain TC-83) (VEEV)	p62,pE2
5	O	Spike glycoprotein E1	polymer	442	47952.1	1	UniProt (P05674) Pfam (PF01589) In PDB	Venezuelan equine encephalitis virus (strain TC-83) (VEEV)	E1 envelope glycoprotein
6	M	CALCIUM ION	non-polymer		40.1	1	Chemie (CA)

Sequence modifications

K: 1 - 275 (UniProt: P05674)

PDB	External Database	Details
Asn 64	Lys 64	engineered mutation

M: 1 - 70 (UniProt: Q86YD5)

PDB	External Database	Details
Gly 71	-	expression tag
Pro 72	-	expression tag
Leu 73	-	expression tag
Glu 74	-	expression tag
Val 75	-	expression tag
Leu 76	-	expression tag
Phe 77	-	expression tag
Gln 78	-	expression tag
Gly 79	-	expression tag
Pro 80	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	141188.1
Non-Polymers*	Number of molecules	1
	Total formula weight	40.1
All*	Total formula weight	141228.2

*Water molecules are not included.