7F8W

Cryo-EM structure of the cholecystokinin receptor CCKBR in complex with gastrin-17 and Gq

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Guanine nucleotide-binding protein G(q) subunit alpha	polymer	353	41353.1	1	UniProt (P50148) Pfam (PF00503)	Homo sapiens (Human)	Guanine nucleotide-binding protein alpha-q
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	351	38744.4	1	UniProt (P62873) Pfam (PF00400)	Homo sapiens (Human)	Transducin beta chain 1
3	C (C)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (Human)	G gamma-I
4	D (D)	scFv16	polymer	268	28621.1	1		Homo sapiens
5	E (E)	Gastrin-17	polymer	17	2098.2	1	Pfam (PF00918) UniProt (by SIFTS) (P01350)	Homo sapiens
6	F (R)	Gastrin/cholecystokinin type B receptor	polymer	465	50524.9	1	UniProt (P32239) Pfam (PF00001)	Homo sapiens (Human)	CCK-B receptor,CCK-BR,Cholecystokinin-2 receptor,CCK2-R

Sequence modifications

A: 23 - 353 (UniProt: P50148)

PDB	External Database	Details
Met 1	-	initiating methionine
Gly 2	-	expression tag
Cys 3	-	expression tag
Thr 4	-	expression tag
Leu 5	-	expression tag
Ser 6	-	expression tag
Ala 7	-	expression tag
Glu 8	-	expression tag
Asp 9	-	expression tag
Lys 10	-	expression tag
Ala 11	-	expression tag
Ala 12	-	expression tag
Val 13	-	expression tag
Glu 14	-	expression tag
Arg 15	-	expression tag
Ser 16	-	expression tag
Lys 17	-	expression tag
Met 18	-	expression tag
Ile 19	-	expression tag
Asp 20	-	expression tag
Arg 21	-	expression tag
Asn 22	-	expression tag

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Leu -1	-	expression tag
Leu 0	-	expression tag
Gln 1	-	expression tag

R: 2 - 418 (UniProt: P32239)

PDB	External Database	Details
Asp -8	-	expression tag
Tyr -7	-	expression tag
Lys -6	-	expression tag
Asp -5	-	expression tag
Asp -4	-	expression tag
Asp -3	-	expression tag
Asp -2	-	expression tag
Gly -1	-	expression tag
Ala 0	-	expression tag
Pro 1	-	expression tag
Glu 419	-	expression tag
Phe 420	-	expression tag
Leu 421	-	expression tag
Glu 422	-	expression tag
Val 423	-	expression tag
Leu 424	-	expression tag
Phe 425	-	expression tag
Gln 426	-	expression tag
Gly 427	-	expression tag
Pro 428	-	expression tag
Trp 429	-	expression tag
Ser 430	-	expression tag
His 431	-	expression tag
Pro 432	-	expression tag
Gln 433	-	expression tag
Phe 434	-	expression tag
Glu 435	-	expression tag
Lys 436	-	expression tag
Gly 437	-	expression tag
Gly 438	-	expression tag
Gly 439	-	expression tag
Ser 440	-	expression tag
Gly 441	-	expression tag
Gly 442	-	expression tag
Gly 443	-	expression tag
Ser 444	-	expression tag
Gly 445	-	expression tag
Gly 446	-	expression tag
Ser 447	-	expression tag
Ala 448	-	expression tag
Trp 449	-	expression tag
Ser 450	-	expression tag
His 451	-	expression tag
Pro 452	-	expression tag
Gln 453	-	expression tag
Phe 454	-	expression tag
Glu 455	-	expression tag
Lys 456	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	169202.9
All*	Total formula weight	169202.9

*Water molecules are not included.