7F83

Crystal Structure of a receptor in Complex with inverse agonist

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Growth hormone secretagogue receptor type 1,Soluble cytochrome b562	polymer	416	47183.2	2	UniProt (Q92847) UniProt (P0ABE7) Pfam (PF00001)	Homo sapiens (human)	GHS-R,GH-releasing peptide receptor,GHRP,Ghrelin receptor,Cytochrome b-562
2	C, D, F, G, H... (A, B)	(2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate	non-polymer		356.5	6	Chemie (OLC)
3	E, J (A, B)	2-(2-methylimidazo[2,1-b][1,3]thiazol-6-yl)-1-[2-[(1R)-5-(6-methylpyrimidin-4-yl)-2,3-dihydro-1H-inden-1-yl]-2,7-diazaspiro[3.5]nonan-7-yl]ethanone	non-polymer		512.7	2	Chemie (1KQ)

Sequence modifications

A, B: 35 - 243 (UniProt: Q92847)

PDB	External Database	Details
Lys 130	Thr 130	engineered mutation
Gln 188	Asn 188	engineered mutation

A, B: 1006 - 1111 (UniProt: P0ABE7)

PDB	External Database	Details
Trp 1012	Met 29	engineered mutation
Ile 1107	His 124	engineered mutation
Leu 1111	Arg 128	engineered mutation

A, B: 253 - 342 (UniProt: Q92847)

PDB	External Database	Details
Gly 1001	-	linker
Gly 1002	-	linker
Thr 1003	-	linker
Thr 1004	-	linker
Met 1005	-	linker
Glu 343	-	expression tag
Asn 344	-	expression tag
Leu 345	-	expression tag
Tyr 346	-	expression tag
Phe 347	-	expression tag
Gln 348	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	94366.4
Non-Polymers*	Number of molecules	8
	Total formula weight	3164.6
All*	Total formula weight	97531.0

*Water molecules are not included.