7F7B

Crystal structure of Non-specific class-C acid phosphatase from Sphingobium sp. RSMS bound to BIS-TRIS at pH 5.5

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Acid phosphatase	polymer	296	31206.9	1	UniProt (A0A197BYF0)	Sphingobium sp. 20006FA
2	B (A)	2-[BIS-(2-HYDROXY-ETHYL)-AMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL	non-polymer		209.2	1	Chemie (BTB)
3	C (A)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
4	D (A)	PHOSPHATE ION	non-polymer		95.0	1	Chemie (PO4)
5	E (A)	water	water		18.0	114	Chemie (HOH)

Sequence modifications

A: 19 - 277 (UniProt: A0A197BYF0)

PDB	External Database	Details
Met -18	-	initiating methionine
Ala -17	-	expression tag
Ser -16	-	expression tag
Trp -15	-	expression tag
Ser -14	-	expression tag
His -13	-	expression tag
Pro -12	-	expression tag
Gln -11	-	expression tag
Phe -10	-	expression tag
Glu -9	-	expression tag
Lys -8	-	expression tag
Gly -7	-	expression tag
Ser -6	-	expression tag
Ser -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
His 1	-	expression tag
Ser 2	-	expression tag
Ser 3	-	expression tag
Gly 4	-	expression tag
Ser 5	-	expression tag
Gly 6	-	expression tag
Gly 7	-	expression tag
Gly 8	-	expression tag
Gly 9	-	expression tag
Gly 10	-	expression tag
Glu 11	-	expression tag
Asn 12	-	expression tag
Leu 13	-	expression tag
Tyr 14	-	expression tag
Phe 15	-	expression tag
Gln 16	-	expression tag
Gly 17	-	expression tag
Ser 18	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	31206.9
Non-Polymers*	Number of molecules	3
	Total formula weight	328.5
All*	Total formula weight	31535.4

*Water molecules are not included.