7F75

Cryo-EM structure of Spx-dependent transcription activation complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, I	DNA-directed RNA polymerase subunit alpha	polymer	314	34842.4	3	UniProt (P20429) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) In PDB	Bacillus subtilis	RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha
2	C	DNA-directed RNA polymerase subunit beta	polymer	1193	133847.9	1	UniProt (P37870) Pfam (PF04560) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) In PDB	Bacillus subtilis	RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta
3	D	DNA-directed RNA polymerase subunit beta'	polymer	1199	134445.0	1	UniProt (P37871) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) In PDB	Bacillus subtilis	RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta'
4	E	DNA-directed RNA polymerase subunit omega	polymer	67	7766.9	1	UniProt (O35011) Pfam (PF01192) In PDB	Bacillus subtilis	RNAP omega subunit,RNA polymerase omega 2 subunit,Transcriptase subunit omega
5	F	RNA polymerase sigma factor SigA	polymer	371	43007.4	1	UniProt (P06224) Pfam (PF03979) Pfam (PF00140) Pfam (PF04542) Pfam (PF04539) Pfam (PF04545) In PDB	Bacillus subtilis	Sigma-43,Sigma-A,RNA polymerase sigma subunit
6	H	DNA-directed RNA polymerase subunit epsilon	polymer	69	8263.4	1	UniProt (O31718) Pfam (PF07288) In PDB	Bacillus subtilis	RNA polymerase auxiliary subunit epsilon subunit,RNAP epsilon subunit,DNA-directed RNA polymerase subunit omega 1,RNA polymerase epsilon subunit,RNA polymerase omega 1 subunit,Transcriptase subunit epsilon,Transcriptase subunit omega 1
7	G	transcriptional regulator Spx	polymer	131	15547.9	1	Pfam (PF03960) UniProt (by SIFTS) (O31602) In PDB	Bacillus subtilis
8	J	trxA promoter DNA-Non template strand	polymer	68	21202.6	1		Bacillus subtilis
9	K	trxA promoter DNA-template strand	polymer	68	20886.4	1		Bacillus subtilis
10	L	DNA-directed RNA polymerase subunit delta	polymer	173	20417.9	1	UniProt (P12464) Pfam (PF05066) In PDB	Bacillus subtilis	RNAP delta factor
11	D	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
12	D	ZINC ION	non-polymer		65.4	2	Chemie (ZN)

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	509912.6
Non-Polymers*	Number of molecules	3
	Total formula weight	155.1
All*	Total formula weight	510067.7

*Water molecules are not included.