7F6I

Cryo-EM structure of human bradykinin receptor BK2R in complex Gq proteins and kallidin

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Bradykinin receptor BK2R	polymer	770	86464.7	1	Pfam (PF00001) UniProt (by SIFTS) (P30411) In PDB	Homo sapiens
2	L	Kallidin	polymer	10	1191.4	1	UniProt (by SIFTS) (P01042) In PDB	Homo sapiens
3	B	Guanine nucleotide-binding protein G(q) subunit alpha	polymer	369	43534.4	1	UniProt (P50148) Pfam (PF00503) In PDB	Homo sapiens (Human)	Guanine nucleotide-binding protein alpha-q
4	C	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	354	39086.6	1	UniProt (P62873) Pfam (PF00400) In PDB	Homo sapiens (Human)	Transducin beta chain 1
5	D	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	81	9242.6	1	UniProt (P59768) Pfam (PF00631) In PDB	Homo sapiens (Human)	G gamma-I
6	A	CHOLESTEROL	non-polymer		386.7	3	Chemie (CLR)

Sequence modifications

B: 2 - 359 (UniProt: P50148)

PDB	External Database	Details
Met -9	-	initiating methionine
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
His 1	-	expression tag
Gln 183	Arg 183	conflict
Leu 209	Gln 209	conflict

C: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -13	-	initiating methionine
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag

D: 2 - 71 (UniProt: P59768)

PDB	External Database	Details
Met -9	-	initiating methionine
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
His 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	179519.8
Non-Polymers*	Number of molecules	3
	Total formula weight	1160.0
All*	Total formula weight	180679.8

*Water molecules are not included.