7F5P
The crystal structure of VyPAL2-C214A, a dead mutant of VyPAL2 peptide asparaginyl ligase in form I
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | Peptide Asparaginyl Ligases | polymer | 289 | 31862.4 | 1 | UniProt (A0A509GV09) Pfam (PF01650) In PDB | Viola philippica | |
2 | B, C, E | alpha-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 586.5 | 3 | In PDB GlyTouCan (G62182OO) | |||
3 | D | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 1 | In PDB GlyTouCan (G42666HT) | |||
4 | F | alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]alpha-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 910.8 | 1 | In PDB GlyTouCan (G21381MC) | |||
5 | water | water | 18.0 | 118 | Chemie (HOH) |
Sequence modifications
A: 43 - 332 (UniProt: A0A509GV09)
PDB | External Database | Details |
---|---|---|
- | Asp 171 | deletion |
Hd0 172 | His 172 | conflict |
Ala 214 | Cys 214 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 1 |
Total formula weight | 31862.4 | |
Branched | Number of molecules | 5 |
Total formula weight | 3094.8 | |
All* | Total formula weight | 34957.3 |