7F5P
The crystal structure of VyPAL2-C214A, a dead mutant of VyPAL2 peptide asparaginyl ligase in form I
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Peptide Asparaginyl Ligases | polymer | 289 | 31862.4 | 1 | UniProt (A0A509GV09) Pfam (PF01650) | Viola philippica | |
| 2 | B, C, E (B, C, E) | alpha-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 586.5 | 3 | In PDB GlyTouCan (G62182OO) | |||
| 3 | D (D) | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 1 | In PDB GlyTouCan (G42666HT) | |||
| 4 | F (F) | alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]alpha-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 910.8 | 1 | In PDB GlyTouCan (G21381MC) | |||
| 5 | G (A) | water | water | 18.0 | 118 | Chemie (HOH) |
Sequence modifications
A: 43 - 332 (UniProt: A0A509GV09)
| PDB | External Database | Details |
|---|---|---|
| - | Asp 171 | deletion |
| Hd0 172 | His 172 | conflict |
| Ala 214 | Cys 214 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 31862.4 | |
| Branched | Number of molecules | 5 |
| Total formula weight | 3094.8 | |
| All* | Total formula weight | 34957.3 |
