7F5G

The crystal structure of RBD-Nanobody complex, DL4 (SA4)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C	Spike glycoprotein	polymer	213	23747.6	2	UniProt (P0DTC2) Pfam (PF09408) In PDB	Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)	S glycoprotein,E2,Peplomer protein
2	B, D	Nanobody DL4	polymer	146	16186.6	2		Vicugna pacos
3	E, F	alpha-L-fucopyranose-(1-3)-[2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)][alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose	branched		716.7	2	In PDB GlyTouCan (G63564LA)
4	D, A, B, C	GLYCEROL	non-polymer		92.1	15	Chemie (GOL)
5	D, A, B, C	ACETATE ION	non-polymer		59.0	11	Chemie (ACT)
6		water	water		18.0	613	Chemie (HOH)

Sequence modifications

A, C: 330 - 531 (UniProt: P0DTC2)

PDB	External Database	Details
Ala 327	-	expression tag
Gly 328	-	expression tag
Ser 329	-	expression tag
Gly 532	-	expression tag
Thr 533	-	expression tag
Leu 534	-	expression tag
Glu 535	-	expression tag
Val 536	-	expression tag
Leu 537	-	expression tag
Phe 538	-	expression tag
Gln 539	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	79868.5
Branched	Number of molecules	2
	Total formula weight	1433.4
Non-Polymers*	Number of molecules	26
	Total formula weight	2030.9
All*	Total formula weight	83332.8

*Water molecules are not included.