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SummaryStructural detailsExperimental detailsFunctional detailsSequence NeighborHistoryDownloads

7F54

Cryo-EM structure of afamelanotide-MC4R-Gs_Nb35 complex

Entity
Entity IDChain IDDescriptionTypeChain lengthFormula weightNumber of moleculesDB Name (Accession)Biological sourceDescriptive keywords
1A
(A)		Isoform Gnas-2 of Guanine nucleotide-binding protein G(s) subunit alpha isoforms shortpolymer38044284.11UniProt (P63092-2)
Pfam (PF00503)
UniProt (by SIFTS) (P63092)		Homo sapiens (Human)Adenylate cyclase-stimulating G alpha protein
2B
(B)		Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1polymer38441729.61UniProt (P62873)
Pfam (PF00400)		Homo sapiens (Human)Transducin beta chain 1
3C
(G)		Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2polymer717861.11UniProt (P59768)
Pfam (PF00631)		Homo sapiens (Human)G gamma-I
4D
(N)		Nanobody35polymer12613711.31synthetic construct
5E
(R)		Melanocortin receptor 4polymer50756053.21UniProt (P32245)
Pfam (PF00001)		Homo sapiens (Human)MC4-R
6F
(L)		afamelanotidepolymer141634.91Pfam (PF00976)
UniProt (by SIFTS) (P01189)		synthetic construct
7G
(R)		CALCIUM IONnon-polymer40.11Chemie (CA)
8H
(R)		waterwater18.04Chemie (HOH)
Sequence modifications
A: 1 - 394 (UniProt: P63092-2)
PDBExternal DatabaseDetails
Asn 54Ser 54engineered mutation
Ala 226Gly 212engineered mutation
Ala 268Glu 254engineered mutation
Lys 271Asn 257engineered mutation
Asp 274Lys 260engineered mutation
Lys 280Arg 266engineered mutation
Asp 284Thr 270engineered mutation
Thr 285Ile 271engineered mutation
B: 2 - 340 (UniProt: P62873)
PDBExternal DatabaseDetails
Met -17-initiating methionine
His -16-expression tag
His -15-expression tag
His -14-expression tag
His -13-expression tag
His -12-expression tag
His -11-expression tag
Leu -10-expression tag
Glu -9-expression tag
Val -8-expression tag
Leu -7-expression tag
Phe -6-expression tag
Gln -5-expression tag
Gly -4-expression tag
Pro -3-expression tag
Gly -2-expression tag
Ser -1-expression tag
Ser 0-expression tag
Gly 1-expression tag
Gly 341-expression tag
Ser 342-expression tag
Ser 343-expression tag
Gly 344-expression tag
Gly 345-expression tag
Gly 346-expression tag
Gly 347-expression tag
Ser 348-expression tag
Gly 349-expression tag
Gly 350-expression tag
Gly 351-expression tag
Gly 352-expression tag
Ser 353-expression tag
Ser 354-expression tag
Gly 355-expression tag
Val 356-expression tag
Ser 357-expression tag
Gly 358-expression tag
Trp 359-expression tag
Arg 360-expression tag
Leu 361-expression tag
Phe 362-expression tag
Lys 363-expression tag
Lys 364-expression tag
Ile 365-expression tag
Ser 366-expression tag
R: 1 - 332 (UniProt: P32245)
PDBExternal DatabaseDetails
Gly -1-expression tag
Pro 0-expression tag
Gly 333-expression tag
Gly 334-expression tag
Ser 335-expression tag
Gly 336-expression tag
Gly 337-expression tag
Ser 338-expression tag
Val 339-expression tag
Phe 340-expression tag
Thr 341-expression tag
Leu 342-expression tag
Glu 343-expression tag
Asp 344-expression tag
Phe 345-expression tag
Val 346-expression tag
Gly 347-expression tag
Asp 348-expression tag
Trp 349-expression tag
Glu 350-expression tag
Gln 351-expression tag
Thr 352-expression tag
Ala 353-expression tag
Ala 354-expression tag
Tyr 355-expression tag
Asn 356-expression tag
Leu 357-expression tag
Asp 358-expression tag
Gln 359-expression tag
Val 360-expression tag
Leu 361-expression tag
Glu 362-expression tag
Gln 363-expression tag
Gly 364-expression tag
Gly 365-expression tag
Val 366-expression tag
Ser 367-expression tag
Ser 368-expression tag
Leu 369-expression tag
Leu 370-expression tag
Gln 371-expression tag
Asn 372-expression tag
Leu 373-expression tag
Ala 374-expression tag
Val 375-expression tag
Ser 376-expression tag
Val 377-expression tag
Thr 378-expression tag
Pro 379-expression tag
Ile 380-expression tag
Gln 381-expression tag
Arg 382-expression tag
Ile 383-expression tag
Val 384-expression tag
Arg 385-expression tag
Ser 386-expression tag
Gly 387-expression tag
Glu 388-expression tag
Asn 389-expression tag
Ala 390-expression tag
Leu 391-expression tag
Lys 392-expression tag
Ile 393-expression tag
Asp 394-expression tag
Ile 395-expression tag
His 396-expression tag
Val 397-expression tag
Ile 398-expression tag
Ile 399-expression tag
Pro 400-expression tag
Tyr 401-expression tag
Glu 402-expression tag
Gly 403-expression tag
Leu 404-expression tag
Ser 405-expression tag
Ala 406-expression tag
Asp 407-expression tag
Gln 408-expression tag
Met 409-expression tag
Ala 410-expression tag
Gln 411-expression tag
Ile 412-expression tag
Glu 413-expression tag
Glu 414-expression tag
Val 415-expression tag
Phe 416-expression tag
Lys 417-expression tag
Val 418-expression tag
Val 419-expression tag
Tyr 420-expression tag
Pro 421-expression tag
Val 422-expression tag
Asp 423-expression tag
Asp 424-expression tag
His 425-expression tag
His 426-expression tag
Phe 427-expression tag
Lys 428-expression tag
Val 429-expression tag
Ile 430-expression tag
Leu 431-expression tag
Pro 432-expression tag
Tyr 433-expression tag
Gly 434-expression tag
Thr 435-expression tag
Leu 436-expression tag
Val 437-expression tag
Ile 438-expression tag
Asp 439-expression tag
Gly 440-expression tag
Val 441-expression tag
Thr 442-expression tag
Pro 443-expression tag
Asn 444-expression tag
Met 445-expression tag
Leu 446-expression tag
Asn 447-expression tag
Tyr 448-expression tag
Phe 449-expression tag
Gly 450-expression tag
Arg 451-expression tag
Pro 452-expression tag
Tyr 453-expression tag
Glu 454-expression tag
Gly 455-expression tag
Ile 456-expression tag
Ala 457-expression tag
Val 458-expression tag
Phe 459-expression tag
Asp 460-expression tag
Gly 461-expression tag
Lys 462-expression tag
Lys 463-expression tag
Ile 464-expression tag
Thr 465-expression tag
Val 466-expression tag
Thr 467-expression tag
Gly 468-expression tag
Thr 469-expression tag
Leu 470-expression tag
Trp 471-expression tag
Asn 472-expression tag
Gly 473-expression tag
Asn 474-expression tag
Lys 475-expression tag
Ile 476-expression tag
Ile 477-expression tag
Asp 478-expression tag
Glu 479-expression tag
Arg 480-expression tag
Leu 481-expression tag
Ile 482-expression tag
Thr 483-expression tag
Pro 484-expression tag
Asp 485-expression tag
Gly 486-expression tag
Ser 487-expression tag
Met 488-expression tag
Leu 489-expression tag
Phe 490-expression tag
Arg 491-expression tag
Val 492-expression tag
Thr 493-expression tag
Ile 494-expression tag
Asn 495-expression tag
Ser 496-expression tag
Gly 497-expression tag
Gly 498-expression tag
Ser 499-expression tag
Leu 500-expression tag
Glu 501-expression tag
Val 502-expression tag
Leu 503-expression tag
Phe 504-expression tag
Gln 505-expression tag
Sequence viewer
Contents of the asymmetric unit
PolymersNumber of chains6
Total formula weight165274.1
Non-Polymers*Number of molecules1
Total formula weight40.1
All*Total formula weight165314.2
*Water molecules are not included.

243911

PDB entries from 2025-10-29

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