7F50

X-ray crystal structure of Y149A mutated Hsp72-NBD in complex with AMPPnP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Heat shock 70 kDa protein 1B	polymer	386	42673.2	1	UniProt (P0DMV9) Pfam (PF00012) In PDB	Homo sapiens (Human)	Heat shock 70 kDa protein 2,HSP70-2,HSP70.2
2	A	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
3	A	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
4	A	PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER	non-polymer		506.2	1	Chemie (ANP)
5		water	water		18.0	328	Chemie (HOH)

Sequence modifications

A: 3 - 380 (UniProt: P0DMV9)

PDB	External Database	Details
Ala 149	Tyr 149	engineered mutation
Leu 381	-	expression tag
Glu 382	-	expression tag
His 383	-	expression tag
His 384	-	expression tag
His 385	-	expression tag
His 386	-	expression tag
His 387	-	expression tag
His 388	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	42673.2
Non-Polymers*	Number of molecules	4
	Total formula weight	590.3
All*	Total formula weight	43263.4

*Water molecules are not included.