7F3V
Crystal structure of YfiH with C107A mutation in complex with endogenous UDP-MurNAc
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B, C, D | Purine nucleoside phosphorylase YfiH | polymer | 251 | 27407.0 | 4 | UniProt (P33644) Pfam (PF02578) In PDB | Escherichia coli | Adenosine deaminase YfiH,Polyphenol oxidase YfiH,S-methyl-5'-thioadenosine phosphorylase YfiH |
2 | A, B, C, D | (2R)-2-{[(2R,3R,4R,5S,6R)-3-(acetylamino)-2-{[(S)-{[(R)-{[(2R,3S,4R,5R)-5-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methoxy}(hydroxy)phosphoryl]oxy}(hydroxy)phosphoryl]oxy}-5-hydroxy-6-(hydroxymethyl)tetrahydro-2H-pyran-4-yl]oxy}propanoic acid | non-polymer | 679.4 | 4 | Chemie (EPZ) | |||
3 | A, B | PHOSPHATE ION | non-polymer | 95.0 | 2 | Chemie (PO4) | |||
4 | C, D | 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL | non-polymer | 122.1 | 2 | Chemie (TRS) | |||
5 | water | water | 18.0 | 838 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 1 - 243 (UniProt: P33644)
PDB | External Database | Details |
---|---|---|
Ala 107 | Cys 107 | engineered mutation |
Leu 244 | - | expression tag |
Glu 245 | - | expression tag |
His 246 | - | expression tag |
His 247 | - | expression tag |
His 248 | - | expression tag |
His 249 | - | expression tag |
His 250 | - | expression tag |
His 251 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 4 |
Total formula weight | 109628.0 | |
Non-Polymers* | Number of molecules | 8 |
Total formula weight | 3151.9 | |
All* | Total formula weight | 112779.9 |