7F3V

Crystal structure of YfiH with C107A mutation in complex with endogenous UDP-MurNAc

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Purine nucleoside phosphorylase YfiH	polymer	251	27407.0	4	UniProt (P33644) Pfam (PF02578)	Escherichia coli	Adenosine deaminase YfiH,Polyphenol oxidase YfiH,S-methyl-5'-thioadenosine phosphorylase YfiH
2	E, G, I, K (A, B, C, D)	(2R)-2-{[(2R,3R,4R,5S,6R)-3-(acetylamino)-2-{[(S)-{[(R)-{[(2R,3S,4R,5R)-5-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methoxy}(hydroxy)phosphoryl]oxy}(hydroxy)phosphoryl]oxy}-5-hydroxy-6-(hydroxymethyl)tetrahydro-2H-pyran-4-yl]oxy}propanoic acid	non-polymer		679.4	4	Chemie (EPZ)
3	F, H (A, B)	PHOSPHATE ION	non-polymer		95.0	2	Chemie (PO4)
4	J, L (C, D)	2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL	non-polymer		122.1	2	Chemie (TRS)
5	M, N, O, P (A, B, C, D)	water	water		18.0	838	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 243 (UniProt: P33644)

PDB	External Database	Details
Ala 107	Cys 107	engineered mutation
Leu 244	-	expression tag
Glu 245	-	expression tag
His 246	-	expression tag
His 247	-	expression tag
His 248	-	expression tag
His 249	-	expression tag
His 250	-	expression tag
His 251	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	109628.0
Non-Polymers*	Number of molecules	8
	Total formula weight	3151.9
All*	Total formula weight	112779.9

*Water molecules are not included.