7F1S

Cryo-EM structure of the apo chemokine receptor CCR5 in complex with Gi

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	R	C-C chemokine receptor type 5	polymer	367	42028.1	1	UniProt (P51681) Pfam (PF00001) In PDB	Homo sapiens (Human)	C-C CKR-5,CC-CKR-5,CCR-5,CCR5,CHEMR13,HIV-1 fusion coreceptor
2	A	Guanine nucleotide-binding protein G(i) subunit alpha-1	polymer	354	40447.1	1	UniProt (P63096) Pfam (PF00503) In PDB	Homo sapiens (Human)	Adenylate cyclase-inhibiting G alpha protein
3	B	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	340	37416.9	1	UniProt (P62873) Pfam (PF00400) In PDB	Homo sapiens (Human)	Transducin beta chain 1
4	C	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631) In PDB	Homo sapiens (Human)	G gamma-I

Sequence modifications

R: 2 - 319 (UniProt: P51681)

PDB	External Database	Details
Gly -1	-	expression tag
Ala 0	-	expression tag
Pro 1	-	expression tag
Asn 163	Gly 163	engineered mutation
Leu 320	-	expression tag
Glu 321	-	expression tag
Val 322	-	expression tag
Leu 323	-	expression tag
Phe 324	-	expression tag
Gln 325	-	expression tag
Gly 326	-	expression tag
Pro 327	-	expression tag
Gly 328	-	expression tag
Ser 329	-	expression tag
Trp 330	-	expression tag
Ser 331	-	expression tag
His 332	-	expression tag
Pro 333	-	expression tag
Gln 334	-	expression tag
Phe 335	-	expression tag
Glu 336	-	expression tag
Lys 337	-	expression tag
Gly 338	-	expression tag
Ser 339	-	expression tag
Gly 340	-	expression tag
Ala 341	-	expression tag
Gly 342	-	expression tag
Ala 343	-	expression tag
Ser 344	-	expression tag
Ala 345	-	expression tag
Gly 346	-	expression tag
Ser 347	-	expression tag
Trp 348	-	expression tag
Ser 349	-	expression tag
His 350	-	expression tag
Pro 351	-	expression tag
Gln 352	-	expression tag
Phe 353	-	expression tag
Glu 354	-	expression tag
Lys 355	-	expression tag
Gly 356	-	expression tag
Ser 357	-	expression tag
Asp 358	-	expression tag
Tyr 359	-	expression tag
Lys 360	-	expression tag
Asp 361	-	expression tag
Asp 362	-	expression tag
Asp 363	-	expression tag
Asp 364	-	expression tag
Lys 365	-	expression tag

A: 1 - 354 (UniProt: P63096)

PDB	External Database	Details
Cys 47	Ser 47	engineered mutation
Thr 202	Gly 202	engineered mutation
Ala 203	Gly 203	engineered mutation
Ala 245	Glu 245	engineered mutation
Ser 326	Ala 326	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	127753.3
All*	Total formula weight	127753.3

*Water molecules are not included.