7F16

Cryo-EM structure of parathyroid hormone receptor type 2 in complex with a tuberoinfundibular peptide of 39 residues and G protein

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	R	Parathyroid hormone 2 receptor	polymer	434	49915.1	1	UniProt (P49190) Pfam (PF02793) Pfam (PF00002) In PDB	Homo sapiens (Human)	PTH2 receptor
2	P	Tuberoinfundibular peptide of 39 residues	polymer	39	4512.2	1	UniProt (Q96A98) Pfam (PF14980) In PDB	Homo sapiens (Human)	TIP39,Parathyroid hormone 2
3	A	Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	polymer	394	45683.4	1	UniProt (P63092) Pfam (PF00503) In PDB	Homo sapiens (Human)	Adenylate cyclase-stimulating G alpha protein
4	B	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	371	40227.0	1	UniProt (P54311) Pfam (PF00400) In PDB	Rattus norvegicus (Rat)	Transducin beta chain 1
5	G	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P63212) Pfam (PF00631) In PDB	Bos taurus (Bovine)	G gamma-I
6	N	Nanobody-35	polymer	140	15343.0	1		Lama glama
7	R	CHOLESTEROL	non-polymer		386.7	4	Chemie (CLR)
8	R	PALMITIC ACID	non-polymer		256.4	6	Chemie (PLM)

Sequence modifications

R: 24 - 442 (UniProt: P49190)

PDB	External Database	Details
Met 9	-	initiating methionine
Lys 10	-	expression tag
Thr 11	-	expression tag
Ile 12	-	expression tag
Ile 13	-	expression tag
Ala 14	-	expression tag
Leu 15	-	expression tag
Ser 16	-	expression tag
Tyr 17	-	expression tag
Ile 18	-	expression tag
Phe 19	-	expression tag
Cys 20	-	expression tag
Leu 21	-	expression tag
Val 22	-	expression tag
Phe 23	-	expression tag

A: 1 - 394 (UniProt: P63092)

PDB	External Database	Details
Asn 54	Ser 54	engineered mutation
Ala 226	Gly 226	engineered mutation
Ala 268	Glu 268	engineered mutation
Lys 271	Asn 271	engineered mutation
Asp 274	Lys 274	engineered mutation
Lys 280	Arg 280	engineered mutation
Asp 284	Thr 284	engineered mutation
Thr 285	Ile 285	engineered mutation

B: 2 - 340 (UniProt: P54311)

PDB	External Database	Details
Met -4	-	initiating methionine
Gly -3	-	expression tag
Ser -2	-	expression tag
Leu -1	-	expression tag
Leu 0	-	expression tag
Gln 1	-	expression tag
Gly 341	-	expression tag
Ser 342	-	expression tag
Ser 343	-	expression tag
Gly 344	-	expression tag
Gly 345	-	expression tag
Gly 346	-	expression tag
Gly 347	-	expression tag
Ser 348	-	expression tag
Gly 349	-	expression tag
Gly 350	-	expression tag
Gly 351	-	expression tag
Gly 352	-	expression tag
Ser 353	-	expression tag
Ser 354	-	expression tag
Gly 355	-	expression tag
Val 356	-	expression tag
Ser 357	-	expression tag
Gly 358	-	expression tag
Trp 359	-	expression tag
Arg 360	-	expression tag
Leu 361	-	expression tag
Phe 362	-	expression tag
Lys 363	-	expression tag
Lys 364	-	expression tag
Ile 365	-	expression tag
Ser 366	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	163541.9
Non-Polymers*	Number of molecules	10
	Total formula weight	3085.2
All*	Total formula weight	166627.0

*Water molecules are not included.