7F0R

Cryo-EM structure of Pseudomonas aeruginosa SutA transcription activation complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	DNA-directed RNA polymerase subunit alpha	polymer	345	38264.3	2	UniProt (O52760) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) In PDB	Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)	RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha
2	C	DNA-directed RNA polymerase subunit beta	polymer	1359	151225.3	1	UniProt (Q51561) Pfam (PF04560) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) In PDB	Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)	RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta
3	D	DNA-directed RNA polymerase subunit beta'	polymer	1412	156038.5	1	UniProt (Q9HWC9) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) In PDB	Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)	RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta'
4	E	DNA-directed RNA polymerase subunit omega	polymer	88	9783.9	1	UniProt (Q9HTM1) Pfam (PF01192) In PDB	Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)	RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega
5	G	Transcriptional factor SutA	polymer	109	11497.4	1	UniProt (Q9HTR9) Pfam (PF20661) In PDB	Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
6	F	RNA polymerase sigma factor RpoS	polymer	342	39361.5	1	UniProt (P45684) Pfam (PF00140) Pfam (PF04542) Pfam (PF04539) Pfam (PF04545) In PDB	Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)	Sigma S,Sigma-38
7	I	DNA (70-MER)	polymer	70	21514.7	1		synthetic construct
8	H	DNA (70-MER)	polymer	70	21648.9	1		synthetic construct
9	D	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
10	D	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)

Sequence modifications

A, B: 1 - 333 (UniProt: O52760)

PDB	External Database	Details
Met -11	-	initiating methionine
Gly -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag

C: 1 - 1357 (UniProt: Q51561)

PDB	External Database	Details
Met -1	-	initiating methionine
Gly 0	-	expression tag

D: 2 - 1399 (UniProt: Q9HWC9)

PDB	External Database	Details
Met 0	-	initiating methionine
Leu 1	-	expression tag
Gly 1400	-	expression tag
Ser 1401	-	expression tag
Gly 1402	-	expression tag
Ser 1403	-	expression tag
Trp 1404	-	expression tag
Ser 1405	-	expression tag
His 1406	-	expression tag
Pro 1407	-	expression tag
Gln 1408	-	expression tag
Phe 1409	-	expression tag
Glu 1410	-	expression tag
Lys 1411	-	expression tag

G: 1 - 105 (UniProt: Q9HTR9)

PDB	External Database	Details
Gly -3	-	expression tag
Ala -2	-	expression tag
Met -1	-	expression tag
Gly 0	-	expression tag

F: 1 - 334 (UniProt: P45684)

PDB	External Database	Details
Leu 335	-	expression tag
Glu 336	-	expression tag
His 337	-	expression tag
His 338	-	expression tag
His 339	-	expression tag
His 340	-	expression tag
His 341	-	expression tag
His 342	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	9
	Total formula weight	487598.7
Non-Polymers*	Number of molecules	3
	Total formula weight	155.1
All*	Total formula weight	487753.8

*Water molecules are not included.