7EY3

Double cysteine mutations in T1 lipase

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Thermostable lipase	polymer	387	43237.2	2	UniProt (Q842J9) In PDB	Geobacillus zalihae
2	A, B	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
3	A, B	CALCIUM ION	non-polymer		40.1	2	Chemie (CA)
4	A, B	SODIUM ION	non-polymer		23.0	2	Chemie (NA)
5	A, B	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL)
6		water	water		18.0	346	Chemie (HOH)

Sequence modifications

A, B: 2 - 388 (UniProt: Q842J9)

PDB	External Database	Details
Cys 2	Ser 30	engineered mutation
Cys 384	Ala 412	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	86474.4
Non-Polymers*	Number of molecules	8
	Total formula weight	327.9
All*	Total formula weight	86802.2

*Water molecules are not included.