7EY2
Bifunctional xylosidase/glucosidase LXYL D300N mutant with intermediate substrate xylose
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D, E... (A, B, C, D, E...) | Beta-D-xylosidase/beta-D-glucosidase | polymer | 805 | 86312.1 | 6 | UniProt (G8GLP2) Pfam (PF00933) Pfam (PF01915) Pfam (PF14310) | Lentinula edodes (Shiitake mushroom Lentinus edodes) | |
| 2 | G, J, M, P, S... (G, J, M, P, S...) | beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 586.5 | 6 | In PDB GlyTouCan (G15407YE) | |||
| 3 | H, N, T, W (H, N, T, W) | alpha-D-mannopyranose-(1-2)-beta-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 1235.1 | 4 | In PDB GlyTouCan (G90815BM) | |||
| 4 | I, R (I, R) | alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 910.8 | 2 | In PDB GlyTouCan (G75436UI) | |||
| 5 | K (K) | alpha-D-mannopyranose-(1-2)-beta-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 1397.2 | 1 | In PDB | |||
| 6 | L, O, U, X (L, O, U, X) | alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 1073.0 | 4 | In PDB GlyTouCan (G60005GQ) | |||
| 7 | Q (Q) | alpha-D-mannopyranose-(1-2)-beta-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 1031.9 | 1 | In PDB | |||
| 8 | AA, BA, EA, FA, GA... (A, B, C, D, E...) | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 22 | Chemie (NAG) | |||
| 9 | CA, HA (A, B) | beta-D-xylofuranose | non-polymer | 150.1 | 2 | Chemie (XYZ) | |||
| 10 | DA (A) | beta-D-mannopyranose | non-polymer | 180.2 | 1 | Chemie (BMA) | |||
| 11 | MA, SA, WA (C, D, E) | Xylitol | non-polymer | 152.1 | 3 | Chemie (XYL) | |||
| 12 | AB, BB, CB, DB, EB... (A, B, C, D, E...) | water | water | 18.0 | 1301 | Chemie (HOH) |
Sequence modifications
A, B, C, D, E, F: 1 - 803 (UniProt: G8GLP2)
| PDB | External Database | Details |
|---|---|---|
| Asn 300 | Asp 300 | engineered mutation |
| His 804 | - | expression tag |
| His 805 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 517872.4 | |
| Branched | Number of molecules | 18 |
| Total formula weight | 17002.3 | |
| Non-Polymers* | Number of molecules | 28 |
| Total formula weight | 5803.4 | |
| All* | Total formula weight | 540678.2 |
