7EY2

Bifunctional xylosidase/glucosidase LXYL D300N mutant with intermediate substrate xylose

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E...	Beta-D-xylosidase/beta-D-glucosidase	polymer	805	86312.1	6	UniProt (G8GLP2) Pfam (PF00933) Pfam (PF01915) Pfam (PF14310) In PDB	Lentinula edodes (Shiitake mushroom Lentinus edodes)
2	G, J, M, P, S...	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	6	In PDB GlyTouCan (G15407YE)
3	H, N, T, W	alpha-D-mannopyranose-(1-2)-beta-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		1235.1	4	In PDB GlyTouCan (G90815BM)
4	I, R	alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		910.8	2	In PDB GlyTouCan (G75436UI)
5	K	alpha-D-mannopyranose-(1-2)-beta-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		1397.2	1	In PDB
6	L, O, U, X	alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		1073.0	4	In PDB GlyTouCan (G60005GQ)
7	Q	alpha-D-mannopyranose-(1-2)-beta-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		1031.9	1	In PDB
8	A, B, C, D, E...	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	22	Chemie (NAG)
9	A, B	beta-D-xylofuranose	non-polymer		150.1	2	Chemie (XYZ)
10	A	beta-D-mannopyranose	non-polymer		180.2	1	Chemie (BMA)
11	C, D, E	Xylitol	non-polymer		152.1	3	Chemie (XYL)
12		water	water		18.0	1301	Chemie (HOH)

Sequence modifications

A, B, C, D, E, F: 1 - 803 (UniProt: G8GLP2)

PDB	External Database	Details
Asn 300	Asp 300	engineered mutation
His 804	-	expression tag
His 805	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	517872.4
Branched	Number of molecules	18
	Total formula weight	17002.3
Non-Polymers*	Number of molecules	28
	Total formula weight	5803.4
All*	Total formula weight	540678.2

*Water molecules are not included.