7EW2
Cryo-EM structure of pFTY720-bound Sphingosine 1-phosphate receptor 3 in complex with Gi protein
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Guanine nucleotide-binding protein G(i) subunit alpha-1 | polymer | 354 | 40415.0 | 1 | UniProt (P63096) Pfam (PF00503) | Homo sapiens (Human) | Adenylate cyclase-inhibiting G alpha protein |
| 2 | B (B) | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 356 | 39141.8 | 1 | UniProt (P62873) Pfam (PF00400) | Homo sapiens (Human) | Transducin beta chain 1 |
| 3 | C (C) | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 71 | 7861.1 | 1 | UniProt (P59768) Pfam (PF00631) | Homo sapiens (Human) | G gamma-I |
| 4 | D (S) | scFV16 | polymer | 266 | 28636.8 | 1 | Homo sapiens (Human) | ||
| 5 | E (R) | Sphingosine 1-phosphate receptor 3 | polymer | 412 | 46435.9 | 1 | UniProt (Q99500) Pfam (PF00001) | Homo sapiens (Human) | S1P receptor 3,S1P3,Endothelial differentiation G-protein coupled receptor 3,Sphingosine 1-phosphate receptor Edg-3,S1P receptor Edg-3 |
| 6 | F (R) | (2~{S})-2-azanyl-4-(4-octylphenyl)-2-[[oxidanyl-bis(oxidanylidene)-$l^{6}-phosphanyl]oxymethyl]butan-1-ol | non-polymer | 387.5 | 1 | Chemie (J89) |
Sequence modifications
B: 2 - 340 (UniProt: P62873)
R: 1 - 378 (UniProt: Q99500)
| PDB | External Database | Details |
|---|---|---|
| Met -15 | - | initiating methionine |
| His -14 | - | expression tag |
| His -13 | - | expression tag |
| His -12 | - | expression tag |
| His -11 | - | expression tag |
| Leu -10 | - | expression tag |
| Glu -9 | - | expression tag |
| Val -8 | - | expression tag |
| Leu -7 | - | expression tag |
| Phe -6 | - | expression tag |
| Gln -5 | - | expression tag |
| Gly -4 | - | expression tag |
| Pro -3 | - | expression tag |
| Gly -2 | - | expression tag |
| Ser -1 | - | expression tag |
| Ser 0 | - | expression tag |
| Gly 1 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Met -33 | - | initiating methionine |
| Lys -32 | - | expression tag |
| Thr -31 | - | expression tag |
| Ile -30 | - | expression tag |
| Ile -29 | - | expression tag |
| Ala -28 | - | expression tag |
| Leu -27 | - | expression tag |
| Ser -26 | - | expression tag |
| Tyr -25 | - | expression tag |
| Ile -24 | - | expression tag |
| Phe -23 | - | expression tag |
| Cys -22 | - | expression tag |
| Leu -21 | - | expression tag |
| Val -20 | - | expression tag |
| Phe -19 | - | expression tag |
| Ala -18 | - | expression tag |
| Asp -17 | - | expression tag |
| Tyr -16 | - | expression tag |
| Lys -15 | - | expression tag |
| Asp -14 | - | expression tag |
| Asp -13 | - | expression tag |
| Asp -12 | - | expression tag |
| Asp -11 | - | expression tag |
| Ala -10 | - | expression tag |
| His -9 | - | expression tag |
| His -8 | - | expression tag |
| His -7 | - | expression tag |
| His -6 | - | expression tag |
| His -5 | - | expression tag |
| His -4 | - | expression tag |
| His -3 | - | expression tag |
| His -2 | - | expression tag |
| His -1 | - | expression tag |
| His 0 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 162490.7 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 387.5 | |
| All* | Total formula weight | 162878.1 |
