7EUQ

Crystal structure of C86H-Y124N-G126H-H196S mutant of N(omega)-hydroxy-L-arginine hydrolase

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	N(omega)-hydroxy-L-arginine amidinohydrolase	polymer	281	30032.7	2	UniProt (D2Z025) Pfam (PF00491)	Streptomyces lavendulae	hydroxyarginase
2	C, D, H, I (A, B)	MANGANESE (II) ION	non-polymer		54.9	4	Chemie (MN)
3	E, F, G, J (A, B)	MAGNESIUM ION	non-polymer		24.3	4	Chemie (MG)
4	K, L (A, B)	water	water		18.0	244	Chemie (HOH)

Sequence modifications

A, B: 1 - 273 (UniProt: D2Z025)

PDB	External Database	Details
His 86	Cys 86	engineered mutation
Asn 124	Tyr 124	engineered mutation
His 126	Gly 126	engineered mutation
Ser 196	His 196	engineered mutation
Leu 274	-	expression tag
Glu 275	-	expression tag
His 276	-	expression tag
His 277	-	expression tag
His 278	-	expression tag
His 279	-	expression tag
His 280	-	expression tag
His 281	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	60065.3
Non-Polymers*	Number of molecules	8
	Total formula weight	317.0
All*	Total formula weight	60382.3

*Water molecules are not included.