7EO2
Cryo-EM of Sphingosine 1-phosphate receptor 1 / Gi complex bound to FTY720p
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | Sphingosine 1-phosphate receptor 1 | polymer | 339 | 38240.7 | 1 | UniProt (P21453) Pfam (PF00001) In PDB | Homo sapiens (Human) | S1P receptor 1,S1P1,Endothelial differentiation G-protein coupled receptor 1,Sphingosine 1-phosphate receptor Edg-1,S1P receptor Edg-1 |
2 | B | Guanine nucleotide-binding protein G(i) subunit alpha-1 | polymer | 354 | 40445.1 | 1 | UniProt (P63096) Pfam (PF00503) In PDB | Homo sapiens (Human) | Adenylate cyclase-inhibiting G alpha protein |
3 | C | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 345 | 37915.5 | 1 | UniProt (P62873) Pfam (PF00400) In PDB | Homo sapiens (Human) | Transducin beta chain 1 |
4 | D | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 71 | 7861.1 | 1 | UniProt (P59768) Pfam (PF00631) In PDB | Homo sapiens (Human) | G gamma-I |
5 | E | scFv16 | polymer | 247 | 26293.3 | 1 | Homo sapiens | ||
6 | A | (2~{S})-2-azanyl-4-(4-octylphenyl)-2-[[oxidanyl-bis(oxidanylidene)-$l^{6}-phosphanyl]oxymethyl]butan-1-ol | non-polymer | 387.5 | 1 | Chemie (J89) |
Sequence modifications
A: 1 - 339 (UniProt: P21453)
B: 1 - 354 (UniProt: P63096)
C: 2 - 340 (UniProt: P62873)
PDB | External Database | Details |
---|---|---|
Trp 133 | Phe 133 | conflict |
PDB | External Database | Details |
---|---|---|
Ala 203 | Gly 203 | conflict |
Ser 326 | Ala 326 | conflict |
PDB | External Database | Details |
---|---|---|
Met -4 | - | initiating methionine |
Gly -3 | - | expression tag |
Ser -2 | - | expression tag |
Leu -1 | - | expression tag |
Leu 0 | - | expression tag |
Gln 1 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 5 |
Total formula weight | 150755.7 | |
Non-Polymers* | Number of molecules | 1 |
Total formula weight | 387.5 | |
All* | Total formula weight | 151143.2 |