7EM8

Crystal structure of the PI5P4Kbeta T201M-2a-ATP complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Phosphatidylinositol 5-phosphate 4-kinase type-2 beta	polymer	393	44959.0	2	UniProt (P78356) Pfam (PF01504) In PDB	Homo sapiens (Human)	1-phosphatidylinositol 5-phosphate 4-kinase 2-beta,Diphosphoinositide kinase 2-beta,Phosphatidylinositol 5-phosphate 4-kinase type II beta,PI(5)P 4-kinase type II beta,PIP4KII-beta,PtdIns(5)P-4-kinase isoform 2-beta
2	A	[(2~{R},3~{S},4~{R},5~{R})-5-[2,6-bis(azanyl)purin-9-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methyl phosphono hydrogen phosphate	non-polymer		442.2	1	Chemie (CX0)
3	A	[[(2~{R},3~{S},4~{R},5~{R})-5-[2,6-bis(azanyl)purin-9-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] phosphono hydrogen phosphate	non-polymer		522.2	1	Chemie (CXC)

Sequence modifications

A, B: 31 - 416 (UniProt: P78356)

PDB	External Database	Details
Gly 24	-	expression tag
Pro 25	-	expression tag
Asn 26	-	expression tag
Cys 27	-	expression tag
Ala 28	-	expression tag
Pro 29	-	expression tag
Gly 30	-	expression tag
Met 201	Thr 201	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	89918.0
Non-Polymers*	Number of molecules	2
	Total formula weight	964.4
All*	Total formula weight	90882.4

*Water molecules are not included.