7EKX
Crystal Structure of the Candida Glabrata Glycogen Debranching Enzyme (W470A E564Q) in complex with maltononaose
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | 4-alpha-glucanotransferase | polymer | 1536 | 175428.4 | 2 | UniProt (Q6FSK0) Pfam (PF14699) Pfam (PF14701) Pfam (PF14702) Pfam (PF06202) | Candida glabrata CBS 138 (Yeast Torulopsis glabrata) | Amylo-alpha-1,6-glucosidase,Dextrin 6-alpha-D-glucosidase,Glycogen debrancher,Glycogen debranching enzyme,Oligo-1,4-1,4-glucantransferase |
| 2 | C (C) | alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose | branched | 504.4 | 1 | In PDB GlyTouCan (G96370VA) | |||
| 3 | D (D) | alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose | branched | 342.3 | 1 | In PDB GlyTouCan (G07411ON) | |||
| 4 | E (E) | alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose | branched | 828.7 | 1 | In PDB GlyTouCan (G50146AM) | |||
| 5 | F (F) | alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose | branched | 1153.0 | 1 | In PDB GlyTouCan (G41288IQ) |
Sequence modifications
A, B: 1 - 1528 (UniProt: Q6FSK0)
| PDB | External Database | Details |
|---|---|---|
| Ala 470 | Trp 470 | engineered mutation |
| Gln 564 | Glu 564 | engineered mutation |
| Leu 1529 | - | expression tag |
| Glu 1530 | - | expression tag |
| His 1531 | - | expression tag |
| His 1532 | - | expression tag |
| His 1533 | - | expression tag |
| His 1534 | - | expression tag |
| His 1535 | - | expression tag |
| His 1536 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 350856.8 | |
| Branched | Number of molecules | 4 |
| Total formula weight | 2828.5 | |
| All* | Total formula weight | 353685.3 |
