7EGN
Crystal structure of the P450 BM3 heme domain mutant F87A in complex with N-imidazolyl-hexanoyl-L-phenylalanine and hydroxylamine
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Bifunctional cytochrome P450/NADPH--P450 reductase | polymer | 465 | 53352.8 | 2 | UniProt (P14779) Pfam (PF00067) | Bacillus megaterium (strain ATCC 14581 / DSM 32 / JCM 2506 / NBRC 15308 / NCIMB 9376 / NCTC 10342 / VKM B-512) | Cytochrome P450(BM-3),Cytochrome P450BM-3,Fatty acid monooxygenase,Flavocytochrome P450 BM3 |
| 2 | C, F (A, B) | PROTOPORPHYRIN IX CONTAINING FE | non-polymer | 616.5 | 2 | Chemie (HEM) | |||
| 3 | D, G (A, B) | (2S)-2-(6-imidazol-1-ylhexanoylamino)-3-phenyl-propanoic acid | non-polymer | 329.4 | 2 | Chemie (IC6) | |||
| 4 | E, H (A, B) | HYDROXYAMINE | non-polymer | 33.0 | 2 | Chemie (HOA) | |||
| 5 | I (B) | GLYCEROL | non-polymer | 92.1 | 1 | Chemie (GOL) | |||
| 6 | J, K (A, B) | water | water | 18.0 | 148 | Chemie (HOH) |
Sequence modifications
A, B: 0 - 455 (UniProt: P14779)
| PDB | External Database | Details |
|---|---|---|
| Gly -1 | - | expression tag |
| Ala 87 | Phe 88 | engineered mutation |
| Leu 456 | - | expression tag |
| Glu 457 | - | expression tag |
| His 458 | - | expression tag |
| His 459 | - | expression tag |
| His 460 | - | expression tag |
| His 461 | - | expression tag |
| His 462 | - | expression tag |
| His 463 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 106705.5 | |
| Non-Polymers* | Number of molecules | 7 |
| Total formula weight | 2049.9 | |
| All* | Total formula weight | 108755.4 |
