7EDC

Crystal structure of mutant tRNA [Gm18] methyltransferase TrmH (E107G) in complex with S-adenosyl-L-methionine from Escherichia coli

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	tRNA (guanosine(18)-2'-O)-methyltransferase	polymer	249	27472.4	1	UniProt (C3SM77) Pfam (PF00588) Pfam (PF12105) UniProt (by SIFTS) (P0AGJ2)	Escherichia coli	tRNA [Gm18] methyltransferase
2	B (A)	S-ADENOSYLMETHIONINE	non-polymer		398.4	1	Chemie (SAM) PubChem (5136) PubChem (34755) PubChem (58633578) PubChem (60038769) PubChem (73897310) PubChem (58766358) PubChem (87224684) PubChem (131697735) PubChem (9865603) PubChem (145712080) PubChem (12312798) PubChem (132988922) PubChem (134692588) PubChem (135030730) PubChem (140459785) PubChem (163046094)
3	C (A)	PHOSPHATE ION	non-polymer		95.0	1	Chemie (PO4) PubChem (1061)
4	D (A)	water	water		18.0	66	Chemie (HOH)

Sequence modifications

A: 1 - 229 (UniProt: C3SM77)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Gly 107	Glu 107	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	27472.4
Non-Polymers*	Number of molecules	2
	Total formula weight	493.4
All*	Total formula weight	27965.8

*Water molecules are not included.