7EDC
Crystal structure of mutant tRNA [Gm18] methyltransferase TrmH (E107G) in complex with S-adenosyl-L-methionine from Escherichia coli
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | tRNA (guanosine(18)-2'-O)-methyltransferase | polymer | 249 | 27472.4 | 1 | UniProt (C3SM77) Pfam (PF00588) Pfam (PF12105) UniProt (by SIFTS) (P0AGJ2) In PDB | Escherichia coli | tRNA [Gm18] methyltransferase |
2 | A | S-ADENOSYLMETHIONINE | non-polymer | 398.4 | 1 | Chemie (SAM) | |||
3 | A | PHOSPHATE ION | non-polymer | 95.0 | 1 | Chemie (PO4) | |||
4 | water | water | 18.0 | 66 | Chemie (HOH) |
Sequence modifications
A: 1 - 229 (UniProt: C3SM77)
PDB | External Database | Details |
---|---|---|
Met -19 | - | initiating methionine |
Gly -18 | - | expression tag |
Ser -17 | - | expression tag |
Ser -16 | - | expression tag |
His -15 | - | expression tag |
His -14 | - | expression tag |
His -13 | - | expression tag |
His -12 | - | expression tag |
His -11 | - | expression tag |
His -10 | - | expression tag |
Ser -9 | - | expression tag |
Ser -8 | - | expression tag |
Gly -7 | - | expression tag |
Leu -6 | - | expression tag |
Val -5 | - | expression tag |
Pro -4 | - | expression tag |
Arg -3 | - | expression tag |
Gly -2 | - | expression tag |
Ser -1 | - | expression tag |
His 0 | - | expression tag |
Gly 107 | Glu 107 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 1 |
Total formula weight | 27472.4 | |
Non-Polymers* | Number of molecules | 2 |
Total formula weight | 493.4 | |
All* | Total formula weight | 27965.8 |