7EC8

Polyethylene terephthalate hydrolyzing lipase PET2 mutant - F105R-E110K

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	LipIAF5-2	polymer	294	31610.2	2	UniProt (C3RYL0)	uncultured bacterium
2	C, D, E, F, G... (A, B)	1,2-ETHANEDIOL	non-polymer		62.1	14	Chemie (EDO)
3	H, R, S (A, B)	SULFATE ION	non-polymer		96.1	3	Chemie (SO4)
4	T, U (A, B)	water	water		18.0	655	Chemie (HOH)

Sequence modifications

A, B: 28 - 308 (UniProt: C3RYL0)

PDB	External Database	Details
Arg 105	Phe 105	engineered mutation
Lys 110	Glu 110	engineered mutation
Glu 309	-	expression tag
Asn 310	-	expression tag
Leu 311	-	expression tag
Tyr 312	-	expression tag
Phe 313	-	expression tag
Gln 314	-	expression tag
Gly 315	-	expression tag
His 316	-	expression tag
His 317	-	expression tag
His 318	-	expression tag
His 319	-	expression tag
His 320	-	expression tag
His 321	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	63220.5
Non-Polymers*	Number of molecules	17
	Total formula weight	1157.1
All*	Total formula weight	64377.6

*Water molecules are not included.