7EBM

W363A mutant of Chitin-specific solute binding protein from Vibrio harveyi in complex with chitobiose.

Replaces:  6LZS

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Peptide ABC transporter, periplasmic peptide-binding protein	polymer	540	61172.9	1	UniProt (D0XC84)	Vibrio harveyi (strain 1DA3)	Chitin specific solute-binding protein
2	B (E)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	1	In PDB GlyTouCan (G42666HT)
3	C (A)	1,2-ETHANEDIOL	non-polymer		62.1	1	Chemie (EDO)
4	D, E (A)	CALCIUM ION	non-polymer		40.1	2	Chemie (CA)
5	F (A)	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
6	G (A)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
7	H (A)	water	water		18.0	261	Chemie (HOH)

Sequence modifications

A: -1 - 530 (UniProt: D0XC84)

PDB	External Database	Details
Ala 363	Trp 393	engineered mutation
Leu 531	-	expression tag
Glu 532	-	expression tag
His 533	-	expression tag
His 534	-	expression tag
His 535	-	expression tag
His 536	-	expression tag
His 537	-	expression tag
His 538	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	61172.9
Branched	Number of molecules	1
	Total formula weight	424.4
Non-Polymers*	Number of molecules	5
	Total formula weight	202.0
All*	Total formula weight	61799.3

*Water molecules are not included.