7EB2

Cryo-EM structure of human GABA(B) receptor-Gi protein complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Guanine nucleotide-binding protein G(i) subunit alpha-1	polymer	354	40414.0	1	UniProt (P63096) Pfam (PF00503)	Homo sapiens (Human)	Adenylate cyclase-inhibiting G alpha protein
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	358	39418.1	1	UniProt (P62873) Pfam (PF00400)	Homo sapiens (Human)	Transducin beta chain 1
3	C (Y)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (Human)	G gamma-I
4	D (N)	ScFv16	polymer	269	28813.0	1		synthetic construct
5	E (C)	Gamma-aminobutyric acid type B receptor subunit 1	polymer	937	105976.3	1	UniProt (Q9UBS5) Pfam (PF01094) Pfam (PF00003) Pfam (PF00084)	Homo sapiens (Human)	Gb1
6	F (D)	Gamma-aminobutyric acid type B receptor subunit 2	polymer	808	91359.4	1	UniProt (O75899) Pfam (PF01094) Pfam (PF00003) Pfam (PF18455)	Homo sapiens (Human)	Gb2,G-protein coupled receptor 51,HG20
7	G (C)	baclofen	non-polymer		213.7	1	Chemie (2C0)
8	H (D)	(3S)-5,7-ditert-butyl-3-oxidanyl-3-(trifluoromethyl)-1-benzofuran-2-one	non-polymer		330.3	1	Chemie (FN0)

Sequence modifications

A: 1 - 354 (UniProt: P63096)

PDB	External Database	Details
Asn 47	Ser 47	engineered mutation
Ala 203	Gly 203	engineered mutation
Ala 245	Glu 245	engineered mutation
Ser 326	Ala 326	engineered mutation

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -17	-	initiating methionine
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Leu -10	-	expression tag
Glu -9	-	expression tag
Val -8	-	expression tag
Leu -7	-	expression tag
Phe -6	-	expression tag
Gln -5	-	expression tag
Gly -4	-	expression tag
Pro -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag

C: 15 - 919 (UniProt: Q9UBS5)

PDB	External Database	Details
Met -1	-	expression tag
Lys 0	-	expression tag
Thr 1	-	expression tag
Ile 2	-	expression tag
Ile 3	-	expression tag
Ala 4	-	expression tag
Leu 5	-	expression tag
Ser 6	-	expression tag
Tyr 7	-	expression tag
Ile 8	-	expression tag
Phe 9	-	expression tag
Cys 10	-	expression tag
Leu 11	-	expression tag
Val 12	-	expression tag
Phe 13	-	expression tag
Ala 14	-	expression tag
Leu 920	-	expression tag
Glu 921	-	expression tag
Val 922	-	expression tag
Leu 923	-	expression tag
Phe 924	-	expression tag
Gln 925	-	expression tag
Gly 926	-	expression tag
Pro 927	-	expression tag
His 928	-	expression tag
His 929	-	expression tag
His 930	-	expression tag
His 931	-	expression tag
His 932	-	expression tag
His 933	-	expression tag
His 934	-	expression tag
His 935	-	expression tag

D: 41 - 819 (UniProt: O75899)

PDB	External Database	Details
Met 12	-	initiating methionine
Lys 13	-	expression tag
Thr 14	-	expression tag
Ile 15	-	expression tag
Ile 16	-	expression tag
Ala 17	-	expression tag
Leu 18	-	expression tag
Ser 19	-	expression tag
Tyr 20	-	expression tag
Ile 21	-	expression tag
Phe 22	-	expression tag
Cys 23	-	expression tag
Leu 24	-	expression tag
Val 25	-	expression tag
Phe 26	-	expression tag
Ala 27	-	expression tag
Asp 28	-	expression tag
Tyr 29	-	expression tag
Lys 30	-	expression tag
Asp 31	-	expression tag
Asp 32	-	expression tag
Asp 33	-	expression tag
Asp 34	-	expression tag
Lys 35	-	expression tag
Gly 36	-	expression tag
Ser 37	-	expression tag
Gly 38	-	expression tag
Gly 39	-	expression tag
Ser 40	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	313842.1
Non-Polymers*	Number of molecules	2
	Total formula weight	544.0
All*	Total formula weight	314386.1

*Water molecules are not included.