7E9H

Cryo-EM structure of Gi-bound metabotropic glutamate receptor mGlu4

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	R, S	Metabotropic glutamate receptor 4	polymer	890	99458.4	2	UniProt (Q14833) Pfam (PF01094) Pfam (PF07562) Pfam (PF00003) In PDB	Homo sapiens (Human)	mGluR4
2	A	Guanine nucleotide-binding protein G(i) subunit alpha-3	polymer	354	40552.1	1	UniProt (P08754) Pfam (PF00503) In PDB	Homo sapiens (Human)	G(i) alpha-3
3	B	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	351	38744.4	1	UniProt (P62873) Pfam (PF00400) In PDB	Homo sapiens (Human)	Transducin beta chain 1
4	C	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631) In PDB	Homo sapiens (Human)	G gamma-I
5	D	scFv16	polymer	257	27409.6	1		Homo sapiens
6	R, S	PHOSPHOSERINE	non-polymer		185.1	2	Chemie (SEP)

Sequence modifications

R, S: 33 - 912 (UniProt: Q14833)

PDB	External Database	Details
Asp 23	-	expression tag
Tyr 24	-	expression tag
Lys 25	-	expression tag
Asp 26	-	expression tag
Asp 27	-	expression tag
Asp 28	-	expression tag
Asp 29	-	expression tag
Gly 30	-	expression tag
Ala 31	-	expression tag
Pro 32	-	expression tag

A: 1 - 354 (UniProt: P08754)

PDB	External Database	Details
Asn 47	Ser 47	engineered mutation
Asp 191	Phe 191	engineered mutation
Ala 203	Gly 203	engineered mutation
Ala 245	Glu 245	engineered mutation
Ser 326	Ala 326	engineered mutation

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Leu -1	-	expression tag
Leu 0	-	expression tag
Gln 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	313483.9
Non-Polymers*	Number of molecules	2
	Total formula weight	370.1
All*	Total formula weight	313854.0

*Water molecules are not included.