7E9H
Cryo-EM structure of Gi-bound metabotropic glutamate receptor mGlu4
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (R, S) | Metabotropic glutamate receptor 4 | polymer | 890 | 99458.4 | 2 | UniProt (Q14833) Pfam (PF01094) Pfam (PF07562) Pfam (PF00003) | Homo sapiens (Human) | mGluR4 |
| 2 | C (A) | Guanine nucleotide-binding protein G(i) subunit alpha-3 | polymer | 354 | 40552.1 | 1 | UniProt (P08754) Pfam (PF00503) | Homo sapiens (Human) | G(i) alpha-3 |
| 3 | D (B) | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 351 | 38744.4 | 1 | UniProt (P62873) Pfam (PF00400) | Homo sapiens (Human) | Transducin beta chain 1 |
| 4 | E (C) | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 71 | 7861.1 | 1 | UniProt (P59768) Pfam (PF00631) | Homo sapiens (Human) | G gamma-I |
| 5 | F (D) | scFv16 | polymer | 257 | 27409.6 | 1 | Homo sapiens | ||
| 6 | G, H (R, S) | PHOSPHOSERINE | non-polymer | 185.1 | 2 | Chemie (SEP) |
Sequence modifications
R, S: 33 - 912 (UniProt: Q14833)
A: 1 - 354 (UniProt: P08754)
B: 2 - 340 (UniProt: P62873)
| PDB | External Database | Details |
|---|---|---|
| Asp 23 | - | expression tag |
| Tyr 24 | - | expression tag |
| Lys 25 | - | expression tag |
| Asp 26 | - | expression tag |
| Asp 27 | - | expression tag |
| Asp 28 | - | expression tag |
| Asp 29 | - | expression tag |
| Gly 30 | - | expression tag |
| Ala 31 | - | expression tag |
| Pro 32 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Asn 47 | Ser 47 | engineered mutation |
| Asp 191 | Phe 191 | engineered mutation |
| Ala 203 | Gly 203 | engineered mutation |
| Ala 245 | Glu 245 | engineered mutation |
| Ser 326 | Ala 326 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Met -10 | - | expression tag |
| His -9 | - | expression tag |
| His -8 | - | expression tag |
| His -7 | - | expression tag |
| His -6 | - | expression tag |
| His -5 | - | expression tag |
| His -4 | - | expression tag |
| Gly -3 | - | expression tag |
| Ser -2 | - | expression tag |
| Leu -1 | - | expression tag |
| Leu 0 | - | expression tag |
| Gln 1 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 313483.9 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 370.1 | |
| All* | Total formula weight | 313854.0 |
