7E8I
Structural insight into BRCA1-BARD1 complex recruitment to damaged chromatin
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | J | DNA (145-MER) | polymer | 145 | 44992.6 | 1 | Homo sapiens | ||
| 2 | I | DNA (145-MER) | polymer | 145 | 44521.4 | 1 | Homo sapiens | ||
| 3 | A, E | Histone H3 | polymer | 135 | 15303.9 | 2 | UniProt (A0A310TTQ1) Pfam (PF00125) UniProt (by SIFTS) (P84233) In PDB | Xenopus laevis (African clawed frog) | |
| 4 | B, F | Histone H4 | polymer | 102 | 11263.2 | 2 | UniProt (P62799) Pfam (PF15511) In PDB | Xenopus laevis (African clawed frog) | |
| 5 | C, G | Histone H2A | polymer | 129 | 13952.2 | 2 | UniProt (Q6AZJ8) Pfam (PF00125) Pfam (PF16211) UniProt (by SIFTS) (P06897) In PDB | Xenopus laevis (African clawed frog) | |
| 6 | D, H | Histone H2B 1.1 | polymer | 122 | 13524.8 | 2 | UniProt (P02281) Pfam (PF00125) In PDB | Xenopus laevis (African clawed frog) | H2B1.1 |
| 7 | K | BRCA1-associated RING domain protein 1 | polymer | 356 | 40145.8 | 1 | UniProt (Q99728) Pfam (PF00023) Pfam (PF00533) In PDB | Homo sapiens (Human) | BARD-1,RING-type E3 ubiquitin transferase BARD1 |
| 8 | L | Polyubiquitin-B | polymer | 86 | 9755.1 | 1 | UniProt (J3QS39) Pfam (PF00240) UniProt (by SIFTS) (P0CG47) In PDB | Homo sapiens (Human) |
Sequence modifications
C, G: 1 - 129 (UniProt: Q6AZJ8)
D, H: 1 - 122 (UniProt: P02281)
K: 425 - 777 (UniProt: Q99728)
L: 1 - 76 (UniProt: J3QS39)
| PDB | External Database | Details |
|---|---|---|
| Cys 15 | Lys 16 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Thr 29 | Ser 33 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Gly 422 | - | expression tag |
| His 423 | - | expression tag |
| Met 424 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly -9 | - | expression tag |
| His -8 | - | expression tag |
| Met -7 | - | expression tag |
| Asp -6 | - | expression tag |
| Tyr -5 | - | expression tag |
| Asp -4 | - | expression tag |
| Ile -3 | - | expression tag |
| Pro -2 | - | expression tag |
| Thr -1 | - | expression tag |
| Thr 0 | - | expression tag |
| Cys 76 | Gly 76 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 12 |
| Total formula weight | 247503.2 | |
| All* | Total formula weight | 247503.2 |
