7E7O

Cryo-EM structure of human ABCA4 in NRPE-bound state

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Retinal-specific phospholipid-transporting ATPase ABCA4	polymer	2317	261143.5	1	UniProt (P78363) Pfam (PF12698) Pfam (PF00005) In PDB	Homo sapiens (Human)	ATP-binding cassette sub-family A member 4,RIM ABC transporter,RIM protein,RmP,Retinal-specific ATP-binding cassette transporter,Stargardt disease protein
2	E, N	beta-D-mannopyranose-(1-3)-[beta-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		910.8	2	In PDB GlyTouCan (G89225LT)
3	A	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	7	Chemie (NAG)
4	A	1,2-Distearoyl-sn-glycerophosphoethanolamine	non-polymer		748.1	3	Chemie (3PE)
5	A	[(2S)-3-[2-[(E)-[(2E,4E,6E,8E)-3,7-dimethyl-9-(2,6,6-trimethylcyclohexen-1-yl)nona-2,4,6,8-tetraenylidene]amino]ethoxy-oxidanyl-phosphoryl]oxy-2-[(Z)-octadec-9-enoyl]oxy-propyl] (Z)-octadec-9-enoate	non-polymer		1010.5	1	Chemie (HZL)

Sequence modifications

A: 1 - 2273 (UniProt: P78363)

PDB	External Database	Details
Met -20	-	expression tag
Ala -19	-	expression tag
Asp -18	-	expression tag
Tyr -17	-	expression tag
Lys -16	-	expression tag
Asp -15	-	expression tag
Asp -14	-	expression tag
Asp -13	-	expression tag
Asp -12	-	expression tag
Lys -11	-	expression tag
Ser -10	-	expression tag
Gly -9	-	expression tag
Pro -8	-	expression tag
Asp -7	-	expression tag
Glu -6	-	expression tag
Val -5	-	expression tag
Asp -4	-	expression tag
Ala -3	-	expression tag
Ser -2	-	expression tag
Gly -1	-	expression tag
Arg 0	-	expression tag
Leu 2274	-	expression tag
Glu 2275	-	expression tag
Gly 2276	-	expression tag
Ser 2277	-	expression tag
Asp 2278	-	expression tag
Glu 2279	-	expression tag
Val 2280	-	expression tag
Asp 2281	-	expression tag
Ala 2282	-	expression tag
Val 2283	-	expression tag
Glu 2284	-	expression tag
Gly 2285	-	expression tag
Ser 2286	-	expression tag
His 2287	-	expression tag
His 2288	-	expression tag
His 2289	-	expression tag
His 2290	-	expression tag
His 2291	-	expression tag
His 2292	-	expression tag
His 2293	-	expression tag
His 2294	-	expression tag
His 2295	-	expression tag
His 2296	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	261143.5
Branched	Number of molecules	2
	Total formula weight	1821.6
Non-Polymers*	Number of molecules	11
	Total formula weight	4803.1
All*	Total formula weight	267768.3

*Water molecules are not included.