7E7D

Cryo-EM structure of the SARS-CoV-2 wild-type S-Trimer from a subunit vaccine candidate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C	Spike glycoprotein,Collagen alpha-1(I) chain	polymer	1520	168164.3	3	UniProt (P0DTC2) UniProt (P02452) Pfam (PF16451) Pfam (PF09408) Pfam (PF19209) Pfam (PF01601) Pfam (PF01391) Pfam (PF01410) In PDB	Severe acute respiratory syndrome coronavirus 2 (2019-nCoV SARS-CoV-2)	S glycoprotein,E2,Peplomer protein,Alpha-1 type I collagen
2	d, e, f, D, g...	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	32	In PDB GlyTouCan (G42666HT)
3	C, A, B	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	19	Chemie (NAG)
4	A, B	9-OCTADECENOIC ACID	non-polymer		282.5	3	Chemie (ELA)

Sequence modifications

A, B, C: 1 - 1211 (UniProt: P0DTC2)

PDB	External Database	Details
Arg 1212	-	linker
Ser 1213	-	linker

A, B, C: 1214 - 1520 (UniProt: P02452)

PDB	External Database	Details
Asn 1277	Asp 1219	variant
Lys 1449	Gln 1391	variant
Ser 1492	Thr 1434	variant

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	504493.0
Branched	Number of molecules	32
	Total formula weight	13580.8
Non-Polymers*	Number of molecules	22
	Total formula weight	5050.3
All*	Total formula weight	523124.2

*Water molecules are not included.