7E70

Time-resolved serial femtosecond crystallography reveals early structural changes in channelrhodopsin: 250 microsecond structure

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Archaeal-type opsin 1,Archaeal-type opsin 2	polymer	356	39692.7	1	UniProt (Q93WP2) UniProt (Q8RUT8) Pfam (PF01036) In PDB	Chlamydomonas reinhardtii (Chlamydomonas smithii)	Channelopsin-1,Chlamyopsin 4 light-gated ion channel,Retinal binding protein,Sensory opsin B
2	E	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	1	In PDB GlyTouCan (G42666HT)
3	A	RETINAL	non-polymer		284.4	1	Chemie (RET)
4	A	(2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate	non-polymer		356.5	9	Chemie (OLC)
5		water	water		18.0	38	Chemie (HOH)

Sequence modifications

A: 1 - 245 (UniProt: Q93WP2)
A: 246 - 348 (UniProt: Q8RUT8)

PDB	External Database	Details
Ser 349	-	expression tag
Ser 350	-	expression tag
Glu 351	-	expression tag
Asp 352	-	expression tag
Leu 353	-	expression tag
Tyr 354	-	expression tag
Phe 355	-	expression tag
Gln 356	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	39692.7
Branched	Number of molecules	1
	Total formula weight	424.4
Non-Polymers*	Number of molecules	10
	Total formula weight	3493.3
All*	Total formula weight	43610.4

*Water molecules are not included.