7E5C

Bacterial prolidase mutant D45W/L225Y/H226L/H343I

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Xaa-Pro dipeptidase	polymer	448	51724.3	4	UniProt (A0A1I7CHQ2)	Pseudoalteromonas lipolytica SCSIO 04301	X-Pro dipeptidase,Imidodipeptidase,Proline dipeptidase,Prolidase
2	E, F, G, H, I... (A, B, C, D)	MANGANESE (II) ION	non-polymer		54.9	8	Chemie (MN)
3	M, N, O, P (A, B, C, D)	water	water		18.0	360	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 440 (UniProt: A0A1I7CHQ2)

PDB	External Database	Details
Trp 45	Asp 45	engineered mutation
Tyr 225	Leu 225	engineered mutation
Leu 226	His 226	engineered mutation
Ile 343	His 343	engineered mutation
Leu 441	-	expression tag
Glu 442	-	expression tag
His 443	-	expression tag
His 444	-	expression tag
His 445	-	expression tag
His 446	-	expression tag
His 447	-	expression tag
His 448	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	206897.3
Non-Polymers*	Number of molecules	8
	Total formula weight	439.5
All*	Total formula weight	207336.8

*Water molecules are not included.