7E4P

Crystal structure of tubulin in complex with Ansamitocin P3

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C	Tubulin alpha-1B chain	polymer	440	48966.3	2	UniProt (P81947) Pfam (PF00091) Pfam (PF03953) In PDB	Bos taurus (Bovine)	Alpha-tubulin ubiquitous,Tubulin K-alpha-1,Tubulin alpha-ubiquitous chain
2	B, D	Tubulin beta-2B chain	polymer	431	48391.4	2	UniProt (Q6B856) Pfam (PF00091) Pfam (PF03953) In PDB	Bos taurus (Bovine)
3	E	Stathmin-4	polymer	138	16282.5	1	UniProt (P63043) Pfam (PF00836) In PDB	Rattus norvegicus (Rat)	Stathmin-like protein B3,RB3
4	F	Tubulin-Tyrosine Ligase	polymer	380	43825.9	1	UniProt (E1BQ43) In PDB	Gallus gallus (Chicken)
5	A, C, D	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	3	Chemie (GTP)
6	A, B, C, D, F	MAGNESIUM ION	non-polymer		24.3	5	Chemie (MG)
7	A, C	CALCIUM ION	non-polymer		40.1	2	Chemie (CA)
8	A	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
9	B	GUANOSINE-5'-DIPHOSPHATE	non-polymer		443.2	1	Chemie (GDP)
10	B	2-(N-MORPHOLINO)-ETHANESULFONIC ACID	non-polymer		195.2	1	Chemie (MES)
11	D	(1S,2S,3S,5S,6S,16Z,18Z,20R,21S)-11-chloro-21-hydroxy-12,20-dimethoxy-2,5,9,16-tetramethyl-8,23-dioxo-4,24-dioxa-9,22-diazatetracyclo[19.3.1.1~10,14~.0~3,5~]hexacosa-10(26),11,13,16,18-pentaen-6-yl 2-methylpropanoate	non-polymer		635.1	1	Chemie (BKF)
12	F	PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER	non-polymer		505.2	1	Chemie (ACP)
13		water	water		18.0	717	Chemie (HOH)

Sequence modifications

F: 1 - 378 (UniProt: E1BQ43)

PDB	External Database	Details
His 379	-	expression tag
His 380	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	254823.9
Non-Polymers*	Number of molecules	15
	Total formula weight	3642.1
All*	Total formula weight	258466.0

*Water molecules are not included.