7E4I

Cryo-EM structure of the yeast mitochondrial SAM-Tom40/Tom5/Tom6 complex at 3.0 angstrom

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Sorting assembly machinery 50 kDa subunit	polymer	485	54544.9	1	UniProt (P53969) Pfam (PF01103)	Saccharomyces cerevisiae S288c (Baker's yeast)	TOB complex 55 kDa subunit
2	B (B)	Sorting assembly machinery 35 kDa subunit	polymer	329	37446.1	1	UniProt (P14693) Pfam (PF10806)	Saccharomyces cerevisiae S288c (Baker's yeast)	Mitochondrial 38 kDa outer membrane protein,TOB complex 38 kDa subunit
3	C (C)	Sorting assembly machinery 37 kDa subunit	polymer	351	40498.8	1	UniProt (P50110) Pfam (PF10568) Pfam (PF11801)	Saccharomyces cerevisiae S288c (Baker's yeast)	MAS37 protein,Mitochondrial 37 kDa outer membrane protein
4	D (D)	Mitochondrial import receptor subunit TOM40	polymer	406	43947.3	1	UniProt (P23644) Pfam (PF01459)	Saccharomyces cerevisiae S288c (Baker's yeast)	Mitochondrial import site protein ISP42,Translocase of outer membrane 40 kDa subunit
5	E (E)	Mitochondrial import receptor subunit TOM5	polymer	52	6196.2	1	UniProt (P80967) Pfam (PF10642)	Saccharomyces cerevisiae S288c (Baker's yeast)	Translocase of outer membrane 5 kDa subunit
6	F (F)	Mitochondrial import receptor subunit TOM6	polymer	62	6481.5	1	UniProt (P33448) Pfam (PF17112)	Saccharomyces cerevisiae S288c (Baker's yeast)	Mitochondrial import site protein ISP6,Translocase of outer membrane 6 kDa subunit

Sequence modifications

A: 2 - 484 (UniProt: P53969)

PDB	External Database	Details
Met 0	-	initiating methionine
Ala 1	-	expression tag

C: 1 - 327 (UniProt: P50110)

PDB	External Database	Details
Leu 328	-	expression tag
Glu 329	-	expression tag
Asp 330	-	expression tag
Tyr 331	-	expression tag
Lys 332	-	expression tag
Asp 333	-	expression tag
His 334	-	expression tag
Asp 335	-	expression tag
Gly 336	-	expression tag
Asp 337	-	expression tag
Tyr 338	-	expression tag
Lys 339	-	expression tag
Asp 340	-	expression tag
His 341	-	expression tag
Asp 342	-	expression tag
Ile 343	-	expression tag
Asp 344	-	expression tag
Tyr 345	-	expression tag
Lys 346	-	expression tag
Asp 347	-	expression tag
Asp 348	-	expression tag
Asp 349	-	expression tag
Asp 350	-	expression tag
Lys 351	-	expression tag

D: 2 - 387 (UniProt: P23644)

PDB	External Database	Details
Met 0	-	initiating methionine
Ala 1	-	expression tag
Leu 388	-	expression tag
Glu 389	-	expression tag
Ser 390	-	expression tag
Ala 391	-	expression tag
Gly 392	-	expression tag
Lys 393	-	expression tag
Pro 394	-	expression tag
Ile 395	-	expression tag
Pro 396	-	expression tag
Asn 397	-	expression tag
Pro 398	-	expression tag
Leu 399	-	expression tag
Leu 400	-	expression tag
Gly 401	-	expression tag
Leu 402	-	expression tag
Asp 403	-	expression tag
Ser 404	-	expression tag
Thr 405	-	expression tag

E: 1 - 50 (UniProt: P80967)

PDB	External Database	Details
Met -1	-	initiating methionine
Ala 0	-	expression tag

F: 2 - 61 (UniProt: P33448)

PDB	External Database	Details
Met 0	-	initiating methionine
Ala 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	189114.7
All*	Total formula weight	189114.7

*Water molecules are not included.