7E4F

Mycobacterium tuberculosis enolase mutant - E204A complex with phosphoenolpyruvate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Enolase	polymer	436	45904.3	1	UniProt (P9WNL1) Pfam (PF03952) Pfam (PF00113) In PDB	Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)	2-phospho-D-glycerate hydro-lyase,2-phosphoglycerate dehydratase
2	A	PHOSPHOENOLPYRUVATE	non-polymer		168.0	1	Chemie (PEP)
3	A	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	2	Chemie (PEG)
4	A	1,2-ETHANEDIOL	non-polymer		62.1	1	Chemie (EDO)
5	A	ACETATE ION	non-polymer		59.0	2	Chemie (ACT)
6	A	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
7		water	water		18.0	143	Chemie (HOH)

Sequence modifications

A: 1 - 429 (UniProt: P9WNL1)

PDB	External Database	Details
Met -6	-	initiating methionine
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
Ala 204	Glu 204	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	45904.3
Non-Polymers*	Number of molecules	7
	Total formula weight	584.7
All*	Total formula weight	46489.1

*Water molecules are not included.