7E33

Serotonin 1E (5-HT1E) receptor-Gi protein complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Guanine nucleotide-binding protein G(i) subunit alpha-1	polymer	354	40414.0	1	UniProt (P63096) Pfam (PF00503) In PDB	Homo sapiens (Human)	Adenylate cyclase-inhibiting G alpha protein
2	B	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	345	37915.5	1	UniProt (P62873) Pfam (PF00400) In PDB	Homo sapiens (Human)	Transducin beta chain 1
3	E	scFv16	polymer	248	26466.5	1		Mus musculus (mouse)
4	G	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631) In PDB	Homo sapiens (Human)	G gamma-I
5	R	Soluble cytochrome b562,5-hydroxytryptamine receptor 1E	polymer	504	57528.4	1	UniProt (P0ABE7) UniProt (P28566) Pfam (PF07361) Pfam (PF00001) In PDB	Escherichia coli	Cytochrome b-562,5-HT-1E,5-HT1E,S31,Serotonin receptor 1E
6	R	3-(1-methylpiperidin-4-yl)-1H-indol-5-ol	non-polymer		230.3	1	Chemie (HVU)

Sequence modifications

A: 1 - 354 (UniProt: P63096)

PDB	External Database	Details
Asn 47	Ser 47	engineered mutation
Ala 203	Gly 203	engineered mutation
Ala 245	Glu 245	engineered mutation
Ser 326	Ala 326	engineered mutation

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -4	-	initiating methionine
Gly -3	-	expression tag
Ser -2	-	expression tag
Leu -1	-	expression tag
Leu 0	-	expression tag
Gln 1	-	expression tag

R: -115 - -10 (UniProt: P0ABE7)

PDB	External Database	Details
Asp -138	-	expression tag
Tyr -137	-	expression tag
Lys -136	-	expression tag
Asp -135	-	expression tag
Asp -134	-	expression tag
Asp -133	-	expression tag
Asp -132	-	expression tag
Ala -131	-	expression tag
Lys -130	-	expression tag
Leu -129	-	expression tag
Gln -128	-	expression tag
Thr -127	-	expression tag
Met -126	-	expression tag
His -125	-	expression tag
His -124	-	expression tag
His -123	-	expression tag
His -122	-	expression tag
His -121	-	expression tag
His -120	-	expression tag
His -119	-	expression tag
His -118	-	expression tag
His -117	-	expression tag
His -116	-	expression tag
Trp -109	Met 29	engineered mutation
Ile -14	His 124	engineered mutation
Leu -10	Arg 128	engineered mutation
Ala -9	-	linker
Ser -8	-	linker
Glu -7	-	linker
Asn -6	-	linker
Leu -5	-	linker
Tyr -4	-	linker
Phe -3	-	linker
Gln -2	-	linker
Gly -1	-	linker
Gly 0	-	linker
Thr 1	-	linker

R: 2 - 365 (UniProt: P28566)

PDB	External Database	Details
Trp 111	Leu 111	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	170185.5
Non-Polymers*	Number of molecules	1
	Total formula weight	230.3
All*	Total formula weight	170415.8

*Water molecules are not included.