7E32
Serotonin 1D (5-HT1D) receptor-Gi protein complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Guanine nucleotide-binding protein G(i) subunit alpha-1 | polymer | 354 | 40414.0 | 1 | UniProt (P63096) Pfam (PF00503) | Homo sapiens (Human) | Adenylate cyclase-inhibiting G alpha protein |
| 2 | B (B) | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 345 | 37915.5 | 1 | UniProt (P62873) Pfam (PF00400) | Homo sapiens (Human) | Transducin beta chain 1 |
| 3 | C (E) | scFv16 | polymer | 248 | 26466.5 | 1 | Mus musculus (mouse) | ||
| 4 | D (G) | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 71 | 7861.1 | 1 | UniProt (P59768) Pfam (PF00631) | Homo sapiens (Human) | G gamma-I |
| 5 | E (R) | Soluble cytochrome b562,5-hydroxytryptamine receptor 1D | polymer | 516 | 57748.2 | 1 | UniProt (P0ABE7) UniProt (P28221) Pfam (PF07361) Pfam (PF00001) | Escherichia coli | Cytochrome b-562,5-HT-1D,5-HT1D,Serotonin 1D alpha receptor,5-HT-1D-alpha,Serotonin receptor 1D |
| 6 | F (R) | SEROTONIN | non-polymer | 176.2 | 1 | Chemie (SRO) | |||
| 7 | G (R) | CHOLESTEROL | non-polymer | 386.7 | 1 | Chemie (CLR) |
Sequence modifications
A: 1 - 354 (UniProt: P63096)
B: 2 - 340 (UniProt: P62873)
R: -115 - -10 (UniProt: P0ABE7)
R: 2 - 377 (UniProt: P28221)
| PDB | External Database | Details |
|---|---|---|
| Asn 47 | Ser 47 | engineered mutation |
| Ala 203 | Gly 203 | engineered mutation |
| Ala 245 | Glu 245 | engineered mutation |
| Ser 326 | Ala 326 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Met -4 | - | initiating methionine |
| Gly -3 | - | expression tag |
| Ser -2 | - | expression tag |
| Leu -1 | - | expression tag |
| Leu 0 | - | expression tag |
| Gln 1 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Asp -138 | - | expression tag |
| Tyr -137 | - | expression tag |
| Lys -136 | - | expression tag |
| Asp -135 | - | expression tag |
| Asp -134 | - | expression tag |
| Asp -133 | - | expression tag |
| Asp -132 | - | expression tag |
| Ala -131 | - | expression tag |
| Lys -130 | - | expression tag |
| Leu -129 | - | expression tag |
| Gln -128 | - | expression tag |
| Thr -127 | - | expression tag |
| Met -126 | - | expression tag |
| His -125 | - | expression tag |
| His -124 | - | expression tag |
| His -123 | - | expression tag |
| His -122 | - | expression tag |
| His -121 | - | expression tag |
| His -120 | - | expression tag |
| His -119 | - | expression tag |
| His -118 | - | expression tag |
| His -117 | - | expression tag |
| His -116 | - | expression tag |
| Trp -109 | Met 29 | engineered mutation |
| Ile -14 | His 124 | engineered mutation |
| Leu -10 | Arg 128 | engineered mutation |
| Ala -9 | - | linker |
| Ser -8 | - | linker |
| Glu -7 | - | linker |
| Asn -6 | - | linker |
| Leu -5 | - | linker |
| Tyr -4 | - | linker |
| Phe -3 | - | linker |
| Gln -2 | - | linker |
| Gly -1 | - | linker |
| Gly 0 | - | linker |
| Thr 1 | - | linker |
| PDB | External Database | Details |
|---|---|---|
| Trp 127 | Leu 127 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 170405.4 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 562.9 | |
| All* | Total formula weight | 170968.2 |
